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Marwa Tammam

Basic information

Name : Marwa Tammam
Title: lecturer of Microbiology and Immunology
Google Schoolar Link: http://scholar.google.com.eg/citations?user=QQdiuOkAAAAJ&hl=en
Personal Info: Dr. Marwa Ali- Tammam, PhD, lecturer of Microbiology and Immunology. Ph.D degree in Microbiology, Universidad Complutense de Madrid (UCM), Spain; MSc in Pharmaceutical Sciences, Universidad Complutense de Madrid ; BSc in Pharmaceutical Sciences, Ain Shams University . View More...

Education

Certificate Major University Year
PhD Pharmaceutical Sciences Universidad Complutense de Madrid 2009
Masters Pharmaceutical Sciences Universidad Complutense de Madrid 2006
Bachelor Pharmaceutical Sciences Ain Shams University 2002

Teaching Experience

Name of Organization Position From Date To Date
Misr International University Lecturer at Microbiology Department 01/01/2011 01/01/2012
Misr International University Teacher Assistant 01/01/2002 01/01/2004

Researches /Publications

Phenotypic and genotypic characterization of Pseudomonas aeruginosa isolates from Egyptian hospitals - 01/1

Marwa Ali Mohamed Tammam

Marwa M. Raafat , Marwa Ali-Tammam and Amal E. Ali1,

01/10/2016

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen and a leading cause of hospitalacquired infections. Characterization of the isolates from different infection sites might help to control infections caused by the pathogen. The aim of the present work is to characterize P. aeruginosa isolates recovered from different clinical specimens at two hospitals in Cairo with regard to their antibiogram, genotypes and virulence factors. The highest antimicrobial resistance pattern was exhibited by isolates from sputum. Resistance rate recorded for sputum samples to different in- use antibiotics was 80, 80-100, 36, 54 and 54% for Penicillins, Cephems, Carbapenems, Aminoglycosides and Fluoroquinolones, respectively. Phenotypic detection of virulence factors in P. aeruginosa isolates included detection of protease, lecithinase, DNase, hemolysin and pyocyanin revealed that, each isolate had at least one virulence factor. Protease and lecithinase were the most commonly detected, where 68 and 66% of the isolates showed positive protease and lecithinase activities respectively. Random amplified polymorphic DNA (RAPD) genotyping using 2 random primers revealed 22 and 14 different genetic profiles. Phylogenetic trees based on genetic distances showed 3 clusters with obvious similarity between some isolates, indicating common sources of infection. No association could be found between clustering pattern of the isolates, their antibiogram and virulence. Key words: Pseudomonas aeruginosa, antibiogram, genotypes, random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR).

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Molecular diagnosis of Entamoeba spp. versus microscopy in the Great Cairo - 01/1

Marwa Ali Mohamed Tammam

Mohamed H. Roshdy, Nour M. Abd El-Kader, Marwa Ali-Tammam, Isabel Fuentes,, Magdy M. Mohamed, Nabila A. El-Sheikh and Jose Miguel Rubio

01/10/2016

Amoebiasis is a human disease produced by Entamoeba histolytica which causes widespread mortality and morbidity worldwide through diarrheal disease and abscess establishment in parenchymal tissues such as liver, lung, and brain. The true prevalence of infection is unknown for most areas of the world due to the difficulty to characterise Entamoeba histolytica versus other non-pathogenic amoebas with identical morphology, as Entamoeba dispar, and Entamoeba moshkovskii. To overcome microscopy misidentification issues, we tested a nested multiplex polymerase chain reaction (PCR) and a real-time PCR on 194 stool samples collected from incoming dysentery patients in Cairo hospitals diagnosed with E. histolytica by microscopy. Nested PCR showed only 20 (10.3%) samples positive to E. histolytica and 17 (8.7%) to E. dispar. The real-time PCR detected only 19 and 11 samples positive to E. histolytica and E. dispar respectively, showing less sensitivity than the nested PCR. The data show that prevalence of E. histolytica in Cairo is lower when specific diagnosis methods are used instead of traditional microscopy, allowing to differentiate between morphologically identical human amoebas species.

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" Ethics in Publishing Seminar" - 01/0

Marwa Ali Mohamed Tammam

organized by Scientific Research Academy,

01/06/2016

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Competitive Research Proposal Writing" - 01/0

Marwa Ali Mohamed Tammam

given by Prof. Galal Hassan, Organized by DAAD, at the premises of Future University in Egypt

01/05/2016

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