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Staff Researches

Miriam Fouad Yousif

Correlation Between Vicia ervilia L. Willd. Antibacterial Activity

Miriam Fouad Yousif Hanna

Mona M. Okba,Kadriya S. El Deeb, Fathy M. Soliman1

Journal of Natural Sciences Research

2016

Context: Plants are important in devising new antibacterial drugs. Unlike several fabaceae seeds, Vicia ervilia L. Willd., is not used up till now for human consumption. Objective: evaluate antibacterial potential of the seeds and correlate it, if any, with its phenolics. Methods: Seeds ethanol (SEE), aqueous (SAE), and methanol (SME) extracts were prepared. Their total phenolic content (TPC) was determined spectrophotometrically. Antibacterial activity against ten pathogenic bacteria (Mycobacterium africanum, M. bovis, M. caprae, M. microti, M. orygis, Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and Salmonella typhimurium) was evaluated using agar well diffusion assay. Also the minimum inhibitory and bactericidal concentrations (MIC & MBC) were also determined. Results: Total phenolic content of SME is significantly (p<0.001) higher than that of SEE (4.8 and 2.5μg/mg gallic acid equivalent respectively). SME and SEE significantly inhibit the growth of all tested strains except M. caprae and P. aeruginosa. V. ervilia L. Conclusion: The seeds significant antibacterial activity was attributed to its phenolics. Keywords: phenolics, antibacterial activity

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Phytochemical and biological studies of Schinus polygamus growing in Egypt

Miriam Fouad Yousif Hanna

Shabana M. M., El Sayed Aly. M.,El Sayed Abeer. M. and Sleem A. A.

International Journal of Traditional and Herbal Medicine.

2013

The leaf ethanol extract of Schinus polygamus (Cav.) Cabrera has evidenced medicinal value as hepatoprotective. It demonstrated inhibitory effects on standard microbes (approximated to) 50% potency of ofloxacin. The same extract evidenced in vitro cytotoxicity on human cell lines viz, liver carcinoma HEPG2, larynx carcinoma HEP2, and colon carcinoma HCT116 when compared to doxorubicin. The leaf ethanol extract of S. polygamus showed variable anti inflammatory, analgesic, and antipyretic activities. Analysis of the hydrolyzed methanol extract of S. polygamus by HPLC allowed the identification of four phenolic acids and five flavonoid aglycones. Gallic acid and chlorogenic acid were identified as major phenolic acids. Major identified flavonoid aglycones were luteolin, kaempferol, quercetin, naringenin, and apigenin. The anti-inflammatory, analgesic, and antimicrobial activities support scientifically the use of S. polygamus in folk medicine for the fore mentioned variable uses. The content of plant constituents as polyphenols of the leaves of S. polygamus justify to some extent the hepatoprotective, anti-inflammatory and analgesic activity with pain tolerance. DNA fingerprinting of the leaves of S. polygamus was carried out as a mean of identification of the genetic profile of the Egyptian plant. Keywords: Schinus polygamus Cabrera; polyphenolics; hepatoprotective activity; antimicrobial activity; cytotoxic effect

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Microscopical, physicochemical and nutritional characterization of three herbal hepatoprotectives

Miriam Fouad Yousif Hanna

Issa M. Y., El Zalabani S. M., El-Askary H. I., Salah El-Dine R.

WJPPS

2014

Plant-based health products play, nowadays, an important role in the global drug market. Despite this constant rise in commercial importance, the processing of herbal drugs lacks reliable scientific assessment and is often performed without strict regulations. To guarantee the quality of the final product, well-defined specification criteria should be established for raw materials before manufacture. The quality and correct identity of crude powdered plants or plant organs can be checked via inspection of sensory, microscopical and physicochemical characteristics. Liver disorders represent a threatening health problem in Egypt. Herbs are frequently used as supportive complementary remedies to alleviate these ailments. The objective of this study was to establish reliable criteria for proper identification of three hepatoprotective herbal drugs viz., seeds of Linum usitatissimum L. (flaxseeds) and Trigonella foenumgraecum L. (fenugreek seeds), and leaves of Rosmarinus officinalis L. (rosemary leaves). The powdered samples were microscopically examined and subjected to proximate, physicochemical and nutritional analysis. The present work will, thus, provide helpful information on the quality of these herbal materials to ensure genuineness, safety and efficacy prior incorporation in pharmaceutical formulations. Key words: Quality control, flax, fenugreek, rosemary, hepatoprotective.

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Macro and micro morphological study of Rudbeckia hirta L. cultivated in Egypt

Miriam Fouad Yousif Hanna

El Hawary S. S., Galal A. E.,and Kirollos F. N.

Al Azhar Third International Conference of Pharmaceutical and Biological Sciences, Air Defence house, Cairo

2003

none

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Vicia ervilia L. seeds newly explored biological activities

Miriam Fouad Yousif Hanna

2. Mona M. Okba, Gehad A. Abdel Jaleel, Kadrya SH. El Deeb & Fathy M. Soliman

Cogent Biology

2017

Within the global context of increasing poverty in the developing countries, natural products are important in devising new drugs. Vicia ervilia L. Willd., unlike several fabaceae seeds, is not used for human consumption till now. We aim to discover any possible medicinal use of the seed. Analgesic, anti-inflammatory, antiulcerogenic and antihyperglycemic activities were evaluated using hot plate, carrageenan-induced rat paw edema, ethanol-induced ulcer model and alloxan-induced diabetes methods, respectively. Antiviral activity was evaluated using Methylthiazol Tetrazolium assay. V. ervilia seeds ethanol (70%) extract had significant anti-inflammatory, analgesic, antiulcerogenic, antihyperglycemic and antiviral activities. It is of excellent choice for treatment of several illnesses in developing countries due to its diverse resource, easy accessibility, affordability and its newly proved significant wide range of biological activities.

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Botanical study, DNA fingerprinting, nutritional values and certrain proximates of Vicia ervilla L.

Miriam Fouad Yousif Hanna

Okba M, El Deeb K, Soliman F.

International Journal of Pharmacy and Pharmaceutical sciences

2014

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BOTANICAL STUDY, DNA FINGERPRINTING, NUTRITIONAL VALUES AND CERTAIN PROXIMATES OF ENTADA RHEEDII SPRENG

Miriam Fouad Yousif Hanna

Int J Pharm Pharm Sci

2013

Entada rheediiSpreng. (Family Fabaceae) seeds are used in Egypt in folk medicine.Macro- and micro-morphological characters of E. rheedii Spreng.seeds imported from India together with that of roots, stems and leaves cultivated in Egypt were presented with the aim of their identification in entire and powdered forms. Cultivation in Egypt gave a climbing plant instead of the huge fruiting trees bordering the Indian Ocean. Plant materials were fixed, freehand sectioned and stained with Safranin. Leaves are compound, bipinnate; their blades exhibit rubiaceous and few ranunculaceous stomata, non-glandular trichomes and dorsiventral mesophyll. The stem upper part is cylindrical with six ridges. The stem has relatively wide pith surrounded by open collateral vascular bundles. Study of Deoxyribonucleic acid (DNA) fingerprinting, total seed protein profiling, nutritional value and certain proximates was carried out in order to contribute to the identification of the plant material.A total of 53 different fragments have been recorded in DNA fingerprinting, produced mainly by (A-19) primer, showing 15 bands ranging from 1.337 Kbp to 0.356 Kbp,Eight bands were recorded in seed total protein banding profile of E. rheedii Spreng seed with molecular weights ranging from 52 to 9 KDa.High levels of Glutamic acid and Phenyl alanine amino acids were determined.Moisture, carbohydrates and ash percentages were 7.35, 16.47 and 2.83 respectively. Evaluation of macroelements (Ca, Na, K and P); and microelement (Fe) revealed that potassium (K) and phosphorous (P) occupied the highest positions (1264 and 1240 mg/100 g seeds respectively) among the macroelements, whereas the micro element Iron (Fe) level was 3.3 mg/100 g seeds.

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Bioactivities, phenolic compounds and in-vitro propagation

Miriam Fouad Yousif Hanna

Bull. Fac. Pharm. Cairo Univ.

2012

The aqueous and alcoholic extracts of the fresh aerial parts of Lippia citriodora Kunth, family Verbenaceae, cultivated in Egypt, exhibited variable antiinflammatory, antipyretic, analgesic and antioxidant properties. Three phenolic compounds; two phenolic acids, dihydrocaffeic acid (1) and 4-hydroxycinnamic acid (3) and a flavonoid glycoside, luteolin-7-O-glucoside (2), were isolated and identified from the ethyl acetate fraction of the plant. The structures of the isolated compounds were elucidated on the basis of spectral analysis. The effects of explant type, method of sterilization and growth regulators on the in-vitro callus formation of L. citriodora were studied. Shoot tips and leaf explants (cut in the midrib region) sterilized by soaking in 0.2% mercuric chloride for 5 min, then washed twice with sterilized distilled water gave callus, on Murashige and Skoog (MS) medium impregnated with 4 mg/L 6-benzyl amino purine (BAP). Spectrophotometric estimation of the total flavonoids showed that the fresh in-vitro formed callus contained 68.4% of the total flavonoids of the fresh aerial parts of the conventional plant.

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Third Scientific Conference of Faculty of Pharmacy, Cairo University,

Miriam Fouad Yousif Hanna

cairo

2012

none

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Bioactive constituents from Harpephyllum caffrum

Miriam Fouad Yousif Hanna

Pharmacognosy Magazine

2011

Background: The leaf ethanol extract of Harpephyllum caffrum Bernh. has evidenced medicinal value due to its hepatoprotective activity. It demonstrated inhibitory effects on test standard microbes approximated to 40% the potency of ofloxacin and fluconazole. The same extract evidenced in vitro cytotoxicity on human cell lines, liver carcinoma HEPG2, larynx carcinoma HEP2, and colon carcinoma HCT116 cell lines when compared to doxorubicin. Materials and Methods: Fractionation of the leaf ethanol extract led to the isolation of the polyphenols, ethyl gallate, and quercetin-3-O-rhamnoside, a hydrocarbon, hendecane, the fatty acid ester, methyl linoleate, and four triterpenoids, betulonic acid, 3-acetyl-methyl betulinate, lupenone and lupeol for the first time, in addition to the previously reported phenol acids and flavonoids, gallic acid, methyl gallate, quercetin, kaempferol, kaempferol-3-O-rhamnoside, kaempferol-3-O-galactoside, apigenin-7-O-glucoside, and quercetin-3-O-arabinoside. Results: The ethanol extract of the fruit of the genetically related Rhus coriaria L., known as sumac, afforded protocatechuic acid, isoquercitrin, and myricetin-3-O-α-L-rhamnoside from the fruits for the first time, in addition to the previously reported phenol acids and flavonoids, gallic acid, methyl gallate, kaempferol, and quercetin. Conclusion: The leaf ethanol extract of H. caffrum Bernh. exhibited variable antiinflammatory, analgesic, and antipyretic activities, besides the hepatoprotective, in vitro cytotoxic and anti-microbial activities. Key words: Harpephyllum caffrum

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Formulation and Evaluation of Two Anti-inflammatory Herbal Gels

Miriam Fouad Yousif Hanna

JBAPN

2011

Two herbal gels expected to produce a topical anti-inflammatory activity were formulated, characterized and evaluated both phytochemically and biologically. The selected active ingredients were two tannin extracts (Q and G) obtained from Acacia nilotica Del. fruits (Qarad) and Quercus infectoria Oliv. galls (Oak gall). The total polyphenol content of the powdered drugs was estimated colorimetrically. The aqueous methanol (50 %) extracts of the investigated samples were individually incorporated, at different concentrations (0.5 - 2 mg/ml), into polyvinyl alcohol (PVA) hydrogels. Gallic acid was used as marker for HPLC standardization of the extracts and determination of drug content in the hydrogels. The anti-inflammatory activity was assessed by measuring the inhibitory effect of the extracts and hydrogels on xylene-induced ear edema in mice.

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Second Scientific Conference of Faculty of Pharmacy, Cairo University

Miriam Fouad Yousif Hanna

Cairo

2010

none

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