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Staff Researches

Miriam Fouad Yousif

Phytochemical and biological studies of Schinus polygamus growing in Egypt

Miriam Fouad Yousif Hanna

Shabana M. M., El Sayed Aly. M.,El Sayed Abeer. M. and Sleem A. A.

IJTHM

2013

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Microscopical, physicochemical and nutritional characterization of three herbal hepatoprotectives

Miriam Fouad Yousif Hanna

Issa M. Y., El Zalabani S. M., El-Askary H. I., Salah El-Dine R.

WJPPS

2014

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Botanical study, DNA fingerprinting, nutritional values and certrain proximates of Vicia ervilla L.

Miriam Fouad Yousif Hanna

Okba M, El Deeb K, Soliman F.

International Journal of Pharmacy and Pharmaceutical sciences

2014

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BOTANICAL STUDY, DNA FINGERPRINTING, NUTRITIONAL VALUES AND CERTAIN PROXIMATES OF ENTADA RHEEDII SPRENG

Miriam Fouad Yousif Hanna

Int J Pharm Pharm Sci

2013

Entada rheediiSpreng. (Family Fabaceae) seeds are used in Egypt in folk medicine.Macro- and micro-morphological characters of E. rheedii Spreng.seeds imported from India together with that of roots, stems and leaves cultivated in Egypt were presented with the aim of their identification in entire and powdered forms. Cultivation in Egypt gave a climbing plant instead of the huge fruiting trees bordering the Indian Ocean. Plant materials were fixed, freehand sectioned and stained with Safranin. Leaves are compound, bipinnate; their blades exhibit rubiaceous and few ranunculaceous stomata, non-glandular trichomes and dorsiventral mesophyll. The stem upper part is cylindrical with six ridges. The stem has relatively wide pith surrounded by open collateral vascular bundles. Study of Deoxyribonucleic acid (DNA) fingerprinting, total seed protein profiling, nutritional value and certain proximates was carried out in order to contribute to the identification of the plant material.A total of 53 different fragments have been recorded in DNA fingerprinting, produced mainly by (A-19) primer, showing 15 bands ranging from 1.337 Kbp to 0.356 Kbp,Eight bands were recorded in seed total protein banding profile of E. rheedii Spreng seed with molecular weights ranging from 52 to 9 KDa.High levels of Glutamic acid and Phenyl alanine amino acids were determined.Moisture, carbohydrates and ash percentages were 7.35, 16.47 and 2.83 respectively. Evaluation of macroelements (Ca, Na, K and P); and microelement (Fe) revealed that potassium (K) and phosphorous (P) occupied the highest positions (1264 and 1240 mg/100 g seeds respectively) among the macroelements, whereas the micro element Iron (Fe) level was 3.3 mg/100 g seeds.

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Bioactivities, phenolic compounds and in-vitro propagation

Miriam Fouad Yousif Hanna

Bull. Fac. Pharm. Cairo Univ.

2012

The aqueous and alcoholic extracts of the fresh aerial parts of Lippia citriodora Kunth, family Verbenaceae, cultivated in Egypt, exhibited variable antiinflammatory, antipyretic, analgesic and antioxidant properties. Three phenolic compounds; two phenolic acids, dihydrocaffeic acid (1) and 4-hydroxycinnamic acid (3) and a flavonoid glycoside, luteolin-7-O-glucoside (2), were isolated and identified from the ethyl acetate fraction of the plant. The structures of the isolated compounds were elucidated on the basis of spectral analysis. The effects of explant type, method of sterilization and growth regulators on the in-vitro callus formation of L. citriodora were studied. Shoot tips and leaf explants (cut in the midrib region) sterilized by soaking in 0.2% mercuric chloride for 5 min, then washed twice with sterilized distilled water gave callus, on Murashige and Skoog (MS) medium impregnated with 4 mg/L 6-benzyl amino purine (BAP). Spectrophotometric estimation of the total flavonoids showed that the fresh in-vitro formed callus contained 68.4% of the total flavonoids of the fresh aerial parts of the conventional plant.

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Third Scientific Conference of Faculty of Pharmacy, Cairo University,

Miriam Fouad Yousif Hanna

cairo

2012

none

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Formulation and Evaluation of Two Anti-inflammatory Herbal Gels

Miriam Fouad Yousif Hanna

JBAPN

2011

Two herbal gels expected to produce a topical anti-inflammatory activity were formulated, characterized and evaluated both phytochemically and biologically. The selected active ingredients were two tannin extracts (Q and G) obtained from Acacia nilotica Del. fruits (Qarad) and Quercus infectoria Oliv. galls (Oak gall). The total polyphenol content of the powdered drugs was estimated colorimetrically. The aqueous methanol (50 %) extracts of the investigated samples were individually incorporated, at different concentrations (0.5 - 2 mg/ml), into polyvinyl alcohol (PVA) hydrogels. Gallic acid was used as marker for HPLC standardization of the extracts and determination of drug content in the hydrogels. The anti-inflammatory activity was assessed by measuring the inhibitory effect of the extracts and hydrogels on xylene-induced ear edema in mice.

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Bioactive constituents from Harpephyllum caffrum

Miriam Fouad Yousif Hanna

Pharmacognosy Magazine

2011

Background: The leaf ethanol extract of Harpephyllum caffrum Bernh. has evidenced medicinal value due to its hepatoprotective activity. It demonstrated inhibitory effects on test standard microbes approximated to 40% the potency of ofloxacin and fluconazole. The same extract evidenced in vitro cytotoxicity on human cell lines, liver carcinoma HEPG2, larynx carcinoma HEP2, and colon carcinoma HCT116 cell lines when compared to doxorubicin. Materials and Methods: Fractionation of the leaf ethanol extract led to the isolation of the polyphenols, ethyl gallate, and quercetin-3-O-rhamnoside, a hydrocarbon, hendecane, the fatty acid ester, methyl linoleate, and four triterpenoids, betulonic acid, 3-acetyl-methyl betulinate, lupenone and lupeol for the first time, in addition to the previously reported phenol acids and flavonoids, gallic acid, methyl gallate, quercetin, kaempferol, kaempferol-3-O-rhamnoside, kaempferol-3-O-galactoside, apigenin-7-O-glucoside, and quercetin-3-O-arabinoside. Results: The ethanol extract of the fruit of the genetically related Rhus coriaria L., known as sumac, afforded protocatechuic acid, isoquercitrin, and myricetin-3-O-α-L-rhamnoside from the fruits for the first time, in addition to the previously reported phenol acids and flavonoids, gallic acid, methyl gallate, kaempferol, and quercetin. Conclusion: The leaf ethanol extract of H. caffrum Bernh. exhibited variable antiinflammatory, analgesic, and antipyretic activities, besides the hepatoprotective, in vitro cytotoxic and anti-microbial activities. Key words: Harpephyllum caffrum

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Second Scientific Conference of Faculty of Pharmacy, Cairo University

Miriam Fouad Yousif Hanna

Cairo

2010

none

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Seasonal Variation in the Essential Oil Composition of Origanum

Miriam Fouad Yousif Hanna

Z. Naturforsch.

2009

The hydrodistilled essential oil content of Origanum majorana L. (Lamiaceae) ranged from 2.5 – 3% with the maximal value (3%) in spring. Analysis of the oil by GC/MS resulted in the identifi cation of 15, 15, 11, and 14 components in the oils prepared in spring, summer, autumn, and winter, respectively. The composition of the essential oils differed quantitatively and qualitatively according to the time of collection. Thymol (38.4%) and cis-sabinene hydrate (25.3%) were the major components in spring plants. Terpinen-4-ol (37.4%, 20.5%, 16.3%) was a major component in the summer, autumn and winter oils, respectively. cis- Sabinene hydrate (54.4%) was major in winter plants while terpinolene (43.1%) was the main component in autumn plants. Other components detected in lower amounts in all oil samples were sabinene and p-cymene (up to 7.4% and 13.9% in autumn), and α-terpinene (up to 13.3% in summer).

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TRITERPENES, PHENOLICS AND HEPATOPROTECTIVE ACTIVITY

Miriam Fouad Yousif Hanna

Bull. Fac. Pharm. Cairo Univ.

2009

Compounds 1-8 were isolated from the aerial parts and roots of Origanum syriacum L. subsp. sinaicum Greuter and Burdet and O. majorana L. They were identified as β-sitosterol 1, four triterpene acids: ursolic acid 2, 3β-hydroxyurs-12-en-27-oic acid 3, 3α-hydroxyolean-12-en-27-oic acid 4, 2α- hydroxyursolic acid 5, a flavone: apigenin 6, a flavonol: quercetin 7 and a phenol acid: rosmarinic acid 8. Identification of these compounds was carried out on bases of physicochemical characters and spectroscopic analyses. The total flavonoid content, as determined colorimetrically, amounted to 0.92 % and 0.87 %, calculated as quercetin per dry weight in both species, respectively. Quantification of flavonoids and phenolic acids was carried out adopting HPLC technique. Major identified components in both species were caffeic, rosmarinic and ferulic acids. Acute toxicity studies showed that O. syriacum L. subsp. sinaicum Greuter and Burdet and O. majorana L. are safe having an LD 50 of up to 10 g/kg b.wt. of aqueous and ethanol extracts and 8.4 and 9.3 g/kg b.wt. of the volatile oils, respectively. Chronic toxicity studies showed safety of both herbs upon long term use. The aqueous and fractionated ethanol extracts of both herbs exhibited a pronounced hepatoprotective, as well as, hepatocurative activity.

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PHARMACOGNOSTICAL AND BIOLOGICAL STUDY OF SPINACH FRUITS

Miriam Fouad Yousif Hanna

Egypt. J. Biomed. Sci.

2009

The fruit of Spinach (Spinacea oleracea L.) was analyzed for its lipoidal, carbohydrate, protein, vitamin and mineral contents, and its biological activities evaluated. The physico-chemical characters of the extracted fixed oil (6.5%) were determined. GLC analysis of the saponifiable fraction of the oil revealed that omega-6 (linoleic acid 20.739%) and omega-9 (oleic acid 33.417%) were the major unsaturated fatty acids; while palmitic (36.258%) and stearic (3.101%) acids were the major saturated ones. In addition, spinasterol, stigmasterol, campesterol, β-sitosterol and α-amyrin, as well as, a series of hydrocarbons were identified in the unsaponifiable fraction. Investigation of the free sugar content and the polysaccharide hydrolysate was carried out by HPLC analysis. Five free sugars were detected and identified as: glucose, sucrose, galactose, fructose and galacturonic acid. The polysaccharide hydrolysate afforded four sugars: galactose, glucose, fructose and galacturonic acid. The amount of crude protein was 17.64 g/100 g; total amino acids amounted to 10.8424 g/100g, among which were 3.3244 g/100 g essential amino acids. Vitamins C, B1, B2, B6, A, D, and E reached 3650 ppm, 236.9 ppm, 38.916 ppm, 5051.3 ppm, 38.272 ppm, 64.543 ppm, and 33.484 ppm respectively. The major minerals were K 686.2 mg/100 g, Mg 206.6 mg/100 g, Fe 69.9 mg/100 g, Mn 6.2 mg/100 g, Zn 2.8 mg/100 g, and, B 1.8 mg/100 g, Cu 1.1 mg/100 g, Cr 0.5 mg/100 g and Co 0.1 mg/ 100 g. The effect of the different fruit extracts, fixed oil and polysaccharide on blood glutathione level was used as a measure to assess its antioxidant activity. The effects of the same fruit extracts on carbon tetrachloride toxicated liver rats were investigated.

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