Author : Mahmoud fathy
CoAuthors : Fathia El-Sharkaw, Zeinab Hassan and Hussein Khaled
Source : Biomedcal sciences
Date of Publication : 08/2014
Abstract :
Background: Matrix metalloproteinases (MMPs) have long been associated with cancer-cell invasion and metastasis.
Few studies are available that describe this association with bladder cancer either related or unrelated to schistosoma
infection.
Evaluating the urinary levels of MMP3 and MMP9 as diagnostic and prognostic biomarkers in different stages of
schistosomal and non schistosomal bladder cancer was the aim of the present study.
Urine samples were collected from 70 patients with schistosomal and non schistosomal bladder cancer at early and
advanced stages and also from12 healthy volunteers as controls. Urinary levels of MMP-3 and MMP-9 was measured by
ELISA technique. Sensitivity and specificity of both markers were determined.
Results: Urinary levels of both MMP-3 and MMP-9 were significantly elevated in all bladder cancer patients compared
with controls. MMP-3 started to elevate in early stages of schistosomal bladder cancer ( 0.173 ng/ml) and non-schistosomal
bladder cancer patients (0.308 ng/ml) compared to control (0.016 ng/ml) and remained elevated in advanced stages (0.166,
0.235 ng/ml) of both types of bladder cancer patients. In contrast, MMP-9 showed a significant elevation in advanced
stages only of both schistosomal and non schistosomal bladder cancer patients (10.33, 21.22 ng/ml) compared
to control (0.409 ng/ml) and this elevation of both markers was much higher in non schistosomal bladder cancer.
Both Metalloproteinases were specific for the diagnosis of the disease but MMP-3 was more sensitive and this
sensitivity was evident in the early stage (84.85% for MMP3, 27.28% for MMP9).
Conclusions: MMP3 may be the recommended urinary metalloproteinases as early diagnostic biomarker in the
early stages of both types of bladder cancer although both MMP9 and MMP3 can be used in the diagnosis of
advanced stages. Further studies are required on large number of urine samples to confirm these results.
Keywords: Bladder cancer, Schistosoma, MMP3,MMP
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