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Dalia Samuel

Basic information

Name : Dalia Samuel
Title: Professor of Pharmaceutical Sciences
Google Schoolar Link: http://scholar.google.com/citations?hl=en&user=_2YgHWoAAAAJ
Personal Info: Prof. Dr. Dalia Samuel, professor of Pharmaceutics at Pharmaceutics and Pharmaceutical Technology Department. her doctoral research was performed at the University of Utah, USA with (distinguished) Prof. William I. Higuchi on transdermal drug delivery. Her post doctorate research was conducted at Oregon State University, Corvallis, Oregon, USA, on transdermal vaccines. View More...

Education

Certificate Major University Year
PhD Pharmaceutical Sciences University of Utah 2003
Masters Pharmaceutical Sciences Helwan University 2000
Bachelor Pharmaceutical Sciences Cairo University 1994

Teaching Experience

Name of Organization Position From Date To Date
FUE, Cairo, Egypt Professor of Pharmaceutics 01/01/2013 01/01/2016
Dept. of Pharmaceutics, Oregon State University, Corvallis, Oregon, USA Post doctorate candidate 01/01/2006 01/01/2007
Dept. of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah, USA PhD student 01/01/2000 01/01/2002
Faculty of Pharmacy, Dept. of Pharmaceutics, Helwan University, Cairo, Egypt TA, Lecturer then Associate Professor of Pharmaceutics 01/01/1995 01/01/2013

Researches /Publications

In situ thermosensitive Tamoxifen citrate loaded hydrogels: an effective tool in breast cancer loco-regional therapy. - 01/0

Dalia Samuel Shaker Kirolos

Dalia S. Shaker, Mohamed A. Shaker, Mahmoud S. Hanafy.

01/07/2016

One of the main challenges for using Tamoxifen citrate (TMC) in breast cancer therapy is achieving proper target and efficient delivery of adequate concentration to the adenocarcinoma without harming healthy glandular and soft fatty tissue. Herein, TMC niosomal thermosensitive hydrogels were proposed as a tool to resolve this challenge. Niosomes were prepared by film hydration technique and incorporated into Pluronics thermosensitive gels prepared using cold method. The prepared hydrogels were evaluated for gelation temperature, rheological behavior and in vitro drug release. Moreover, in vivo anti-tumor activity was examined in Ehrlich carcinoma mice model through reporting solid tumor volume regression and tissue distribution of TMC. Type and ratio of used poloxamers were manipulated to provide the optimal gelation temperature (34e37 C). Rheological analysis showed low viscosity and elasticity values at low and room temperature while these values significantly increased at the physiological temperature. A prolonged diffusion-driven release of TMC was detected. In vivo data showed, evidently, that anticancer activity was improved with significant retention of the drug at the tumor site. These encouraging results confined that this in situ hydrogel depot offers an attractive approach for controlled delivery of TMC and clinically expected to be useful delivery system in loco-regional therapy for breast cancer.

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loco-regional breast cancer therapy through in situ thermosensitive Tamoxifen citrate niosomal gels. - 01/0

Dalia Samuel Shaker Kirolos

Dalia Samuel Shaker, Mohamed A. Shaker, Mahmoud S. Hanafy

01/03/2016

Loco-regional delivery of Tamoxifen Citrate (TMC) is used in this study to localize its activity into the vicinity of tumor and hence improving therapeutic outcome with less toxicity on other organs. Herein, innovative TMC niosomal thermosensitive gels were proposed as a tool to achieve this goal. Niosomes were prepared by thin lipid film hydration technique and evaluated for cellular uptake and cytotoxicity. The anti-cancer activity was also tested in-vitro using MCF-7 breast cancer cell line. Moreover, in-vivo anti-tumor efficacy was examined in Ehrlich carcinoma mice model through reporting solid tumor volume regression and tissue TMC distribution. Significantly enhanced cellular uptake (2.8 fold) and greater cytotoxic activity with MCF-7 breast cancer cell line were obtained from vesicles prepared with span 60: cholesterol (1:1 molar ratio). Niosomes were then packed in thermosensitive gels using cold method. TMC niosomal thermosensitive gels were evaluated for gelation temperature, rheological behavior and in vitro drug release. Type and ratio of used poloxamers were manipulated to provide an optimal gelation temperature (34-37°C). Rheological analysis showed low viscosity and elasticity values at low temperature while these values significantly increased at elevated physiological temperature. A prolonged release of TMC following a diffusion-driven release model was detected. Furthermore, in vivo data showed evidently that anticancer activity was improved with significant retention of the drug at the tumor site. These encouraging results confined that this in situ gel depot offers an attractive approach for controlled delivery of TMC and clinically expected to be useful candidate in breast cancer loco-regional therapy.

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Cellular uptake, cytotoxicity and in-vivo evaluation of Tamoxifen citrate loaded niosomes - 01/0

Dalia Samuel Shaker Kirolos

Dalia S. Shaker, Mohamed A. Shaker, Mahmoud S. Hanafy.

01/07/2015

One of the main challenges in Tamoxifen cancer therapy is achieving localized, efficient and sustained delivery without harming normal healthy organs. This study focused on evaluating Tamoxifen Citrate (TMC) niosomes for localized cancer therapy through in-vitro breast cancer cytotoxicity as well as in-vivo solid anti-tumor efficacy. Different niosomal formulae were prepared by film hydration technique and characterized for entrapment efficiency % (E. E), vesicle size, morphology, and in-vitro release. The cellular uptake and anti-cancer activity were also tested in-vitro using MCF-7 breast cancer cell line. Moreover, in-vivo anti-tumor efficacy was examined in Ehrlich carcinoma mice model through reporting solid tumor volume regression and tissue TMC distribution. The obtained niosomes prepared with Span 60: cholesterol (1: 1 molar ratio) showed a distinct nano-spherical shape with EE up to 92.3% ± 2.3. Remarkably prolonged release of TMC following diffusion release behavior was detected. The optimized formula showed significantly enhanced cellular uptake (2.8 fold) and exhibited significantly greater cytotoxic activity with MCF-7 breast cancer cell line. In-vivo experiment showed enhanced tumor volume reduction of niosomal TMC when compared to free TMC. Based on these results, the prepared niosomes demonstrated to be promising as a nano-size delivery vehicle for localized and sustained TMC cancer therapy.

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In-Vitro Cellular Uptake and Cytotoxicity of Tamoxifen Citrate Niosomes. - 01/0

Dalia Samuel Shaker Kirolos

Dalia S. Shaker, Mohamed A. Shaker, Mahmoud S. Hanafy

01/02/2015

Objective: This study focused on evaluating Tamoxifen Citrate (TMC) niosomes compared to free TMC regarding In-Vitro cellular uptake and cytotoxicity. Methods: Different niosomal formulae were prepared by thin film hydration technique and evaluated for entrapment efficiency % (E.E. %), vesicle size, vesicular morphology, DSC, in-vitro release and the optimized formula was assessed for its in-vitro cellular uptake and cytotoxicity. Results: The niosomal formulation composed of span 60: cholesterol (1:1 molar ratio) using lipid concentration 1% (w/v) showed the highest E.E. % (92.3 %) and exhibited significantly (P<0.05) lower vesicular size (200 nm) with a distinct nano-spherical shape. DSC revealed the conversion of crystalline TMC to amorphous form. Remarkably prolonged release of TMC for 6 days following Fickian diffusion release behavior was detected .The optimized formula showed significantly enhanced cellular uptake in MCF-7 breast cancer model cell line. However three days cytotoxicity study showed insignificant difference between niosomal TMC and free TMC regarding lethal effect. Despite our finding that only 60% of TMC released from niosomes and became available for the cells to exhibit similar lethal effect to free TMC, after 3 days of the experiment, which represents the maximum experimental time for cytotoxicity study. Conclusion: The optimized formula could contribute in increasing cellular uptake and cytotoxicity.

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Novel Approaches for Promoting Drug and Gene Transdermal Permeation - 01/0

Dalia Samuel Shaker Kirolos

Dalia S Shaker

01/04/2014

Objective: This study focused on evaluating Tamoxifen Citrate (TMC) niosomes compared to free TMC regarding In-Vitro cellular uptake and cytotoxicity. Methods: Different niosomal formulae were prepared by thin film hydration technique and evaluated for entrapment efficiency % (E.E. %), vesicle size, vesicular morphology, DSC, in-vitro release and the optimized formula was assessed for its in-vitro cellular uptake and cytotoxicity. Results: The niosomal formulation composed of span 60: cholesterol (1:1 molar ratio) using lipid concentration 1% (w/v) showed the highest E.E. % (92.3 %) and exhibited significantly (P<0.05) lower vesicular size (200 nm) with a distinct nano-spherical shape. DSC revealed the conversion of crystalline TMC to amorphous form. Remarkably prolonged release of TMC for 6 days following Fickian diffusion release behavior was detected .The optimized formula showed significantly enhanced cellular uptake in MCF-7 breast cancer model cell line. However three days cytotoxicity study showed insignificant difference between niosomal TMC and free TMC regarding lethal effect. Despite our finding that only 60% of TMC released from niosomes and became available for the cells to exhibit similar lethal effect to free TMC, after 3 days of the experiment, which represents the maximum experimental time for cytotoxicity study. Conclusion: The optimized formula could contribute in increasing cellular uptake and cytotoxicity.

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Awards

Award Donor Date
Editorial Board member of Future Journal of Pharmaceutical Sciences, Elsevier FUE 2015
AAPS membership AAPS organization, USA 2000

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