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Staff Researches

Marwa Tammam

" Genotyping and Subgenotyping analysis of cryptosporiodiosis infection in Egypt".

Marwa Ali Mohamed Tammam

Nour M. Abd El Kader, Abd El Rahman B. Abd El Ghaffar, Marwa A Tammam, José M Rubio, Ahmed Osman, and Nabila El Sheikh.

Journal of Molecular Diagnosis and Vaccines. Vol. 12, 2014.

2014

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Molecular diagnosis of Entamoeba spp. versus microscopy in the Great Cairo

Marwa Ali Mohamed Tammam

Mohamed H. Roshdy, Nour M. Abd El-Kader, Marwa Ali-Tammam, Isabel Fuentes,, Magdy M. Mohamed, Nabila A. El-Sheikh and Jose Miguel Rubio

Acta Parasitologica

2016

Amoebiasis is a human disease produced by Entamoeba histolytica which causes widespread mortality and morbidity worldwide through diarrheal disease and abscess establishment in parenchymal tissues such as liver, lung, and brain. The true prevalence of infection is unknown for most areas of the world due to the difficulty to characterise Entamoeba histolytica versus other non-pathogenic amoebas with identical morphology, as Entamoeba dispar, and Entamoeba moshkovskii. To overcome microscopy misidentification issues, we tested a nested multiplex polymerase chain reaction (PCR) and a real-time PCR on 194 stool samples collected from incoming dysentery patients in Cairo hospitals diagnosed with E. histolytica by microscopy. Nested PCR showed only 20 (10.3%) samples positive to E. histolytica and 17 (8.7%) to E. dispar. The real-time PCR detected only 19 and 11 samples positive to E. histolytica and E. dispar respectively, showing less sensitivity than the nested PCR. The data show that prevalence of E. histolytica in Cairo is lower when specific diagnosis methods are used instead of traditional microscopy, allowing to differentiate between morphologically identical human amoebas species.

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Phenotypic and genotypic characterization of Pseudomonas aeruginosa isolates from Egyptian hospitals

Marwa Ali Mohamed Tammam

Marwa M. Raafat , Marwa Ali-Tammam and Amal E. Ali1,

African Journal of Microbiology Research

2016

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen and a leading cause of hospitalacquired infections. Characterization of the isolates from different infection sites might help to control infections caused by the pathogen. The aim of the present work is to characterize P. aeruginosa isolates recovered from different clinical specimens at two hospitals in Cairo with regard to their antibiogram, genotypes and virulence factors. The highest antimicrobial resistance pattern was exhibited by isolates from sputum. Resistance rate recorded for sputum samples to different in- use antibiotics was 80, 80-100, 36, 54 and 54% for Penicillins, Cephems, Carbapenems, Aminoglycosides and Fluoroquinolones, respectively. Phenotypic detection of virulence factors in P. aeruginosa isolates included detection of protease, lecithinase, DNase, hemolysin and pyocyanin revealed that, each isolate had at least one virulence factor. Protease and lecithinase were the most commonly detected, where 68 and 66% of the isolates showed positive protease and lecithinase activities respectively. Random amplified polymorphic DNA (RAPD) genotyping using 2 random primers revealed 22 and 14 different genetic profiles. Phylogenetic trees based on genetic distances showed 3 clusters with obvious similarity between some isolates, indicating common sources of infection. No association could be found between clustering pattern of the isolates, their antibiogram and virulence. Key words: Pseudomonas aeruginosa, antibiogram, genotypes, random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR).

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“Bioinformatics Workshop”

Marwa Ali Mohamed Tammam

“Bioinformatics Workshop” organized by Department of Microbiology& Immunology, Faculty of Pharmaceutical Sciences& Pharmaceutical Industries. Future University in Egypt ( FUE).

16-17th of September, 2015, Cairo, Egypt.

2015

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" Ethics in Publishing Seminar"

Marwa Ali Mohamed Tammam

organized by Scientific Research Academy,

2016

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Competitive Research Proposal Writing"

Marwa Ali Mohamed Tammam

given by Prof. Galal Hassan, Organized by DAAD, at the premises of Future University in Egypt

2016

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"Genetic Diversity of Clinical Pseudomonas aeruginosa Isolates from Egyptian Hospitals".

Marwa Ali Mohamed Tammam

Poster: Marwa M. Raafat, Amal E. Ali

3rd FUE International Conference of Pharmaceutical Sciences, Cairo, Egypt,

2015

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"Intestinal Ameabiasis in Egypt is the situation correctly evaluated?"

Marwa Ali Mohamed Tammam

3rd FUE International Conference of Pharmaceutical Sciences, Cairo, Egypt, 9th -11th February 2015.

2015

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XVIII International Congress For Tropical Medicine and Malaria,

Marwa Ali Mohamed Tammam

Rio de Janiero, Brazil,

2012

Poster: Genotyping and subgenotyping analysis of cryptosporidiosis infection in Egypt

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VII European Congress on Tropical Medicine and International Health.

Marwa Ali Mohamed Tammam

Barcelona, Spain,

2011

Poster: Genotype characterization of Giardia duodenalis in human isolates from Egypt

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XX Congreso Latinoamericano de Parasitolog?a and XV Congreso Colombiano de Parasitolog?a y Medicina Tropical,

Marwa Ali Mohamed Tammam

Begota, Colombia

2011

Symposium: JM Rubio, M.A. Tammam, Thuy H. Ta Tang. “Uso de PCR m?ltiple en el diagn?stico simultaneo de par?sitos”. (The use of multiple PCR for simultaneous diagnosis of parasites).

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