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Staff Researches

Dalia Samuel

Optimization of Declofinac sodium loaded chitosan-microspheres prepared by a modified coacervation method

Dalia Samuel Shaker Kirolos

E. S. Ibrahim, D. S. Shaker, M. K. Ghorab, R. S. Abdel Rashid

the American Association of Pharmaceutical Scientists AAPS. Los Angeles, CA, USA

2009

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Silicone polymer uptake method ones concentration in micelle systems used in skin transport studies,

Dalia Samuel Shaker Kirolos

K. S. Warner, N. He, S. K. Li, A-H. Ghanem, D. S. Shaker, W. I. Higuchi

the American Association of Pharmaceutical Scientists AAPS. Salt Lake City, Utah, USA

2002

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Formulation and Pharmacotechnical evaluation of transdermal delivery patches of Lornoxicam.

Dalia Samuel Shaker Kirolos

M. El Nabarawi, D. Shaker, S. Youssef.

The American Association of Pharmaceutical Scientists AAPS.Washington D. C., USA

2011

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In-vitro skin permeation and anti-inflammatory effect of Lornoxicam transdermal patches using rat paw edema model

Dalia Samuel Shaker Kirolos

M. El Nabarawi, D. Shaker, S. Youssef

The American Association of Pharmaceutical Scientists AAPS. Washington D. C., USA

2011

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Mechanistic Studies of the Effect of Cyclodextrins on the in-vitro Transdermal Permeation of Corticosterone through Hairless Mouse Skin

Dalia Samuel Shaker Kirolos

D. S. Shaker, A-H. Ghanem, S. K. Li, K. S. Warner, F. M. Hashem, W. I. Higuchi

the American Association of Pharmaceutical Scientists, Denver, Colorado, USA

2001

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Attenuated Mycobacterium marinum protects zebrafish against mycobacteriosis

Dalia Samuel Shaker Kirolos

J. Fish Dis

2009

A special transdermal vaccine was prepared to protect fish

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In Vitro and In Vivo evaluation of combined time and pH- dependent oral colonic targeted prednisolone Microspheres.

Dalia Samuel Shaker Kirolos

British Journal of Pharmaceutical research BJPR.

2013

Aims: to enhance the anti-inflammatory effect as well as oral absorption of prednisolone (PR), through formulation of colonic targeted microspheres prepared from a blend of time and pH- dependent polymers and loaded with PR. Study Design: In Vitro and In Vivo Evaluation of Combined Time and pH- Dependent Oral Colonic Targeted Prednisolone Microspheres. Methodology: Microspheres were prepared by solvent evaporation method using different ethyl cellulose (EC) and Eudragit® S-100 (ES100) ratios with 0.5 and 1% w/v span® 80 as emulsifier. The microspheres were evaluated for surface morphology, particle size, drug encapsulation efficiency % and in vitro drug release at pH 1.2 and 7.4. The antiinflammatory activity of selected formula was compared to that of conventional PR tablets. Results: A decrease in drug entrapment efficiency % was obtained with increasing both polymers and surfactant concentrations. Based on drug release results, the formula of 1: Research Article ………… Article British Journal of Pharmaceutical Research, 3(3): 420-434, 2013 421 1: 0.16 w/w/w, EC: ES100: PR ratio with 1% w/v span® 80 was selected for further histopathological evaluation of the anti-inflammatory activity in colitis induced-rats. Histopathological study showed undefined tissue necrosis after treatment with the selected microspheres; however, diffused necrosis was observed in rats treated with the commercial tablets. In vivo absorption study showed that values of Cmax and AUC0-24 of both formulations were insignificantly different. However, the occurrence of Cmax of microspheres was significantly delayed in comparison to free drug (9.17 to 2.67hr) (P<.001). Conclusion: This study has supplied us with brightening results concerning the therapeutic efficacy of a blend of time and pH- dependent polymers colonic targeted microspheres.

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In situ thermosensitive Tamoxifen citrate loaded hydrogels: an effective tool in breast cancer loco-regional therapy.

Dalia Samuel Shaker Kirolos

Dalia S. Shaker, Mohamed A. Shaker, Mahmoud S. Hanafy.

J Drug Delivery Sci Tech.,

2016

One of the main challenges for using Tamoxifen citrate (TMC) in breast cancer therapy is achieving proper target and efficient delivery of adequate concentration to the adenocarcinoma without harming healthy glandular and soft fatty tissue. Herein, TMC niosomal thermosensitive hydrogels were proposed as a tool to resolve this challenge. Niosomes were prepared by film hydration technique and incorporated into Pluronics thermosensitive gels prepared using cold method. The prepared hydrogels were evaluated for gelation temperature, rheological behavior and in vitro drug release. Moreover, in vivo anti-tumor activity was examined in Ehrlich carcinoma mice model through reporting solid tumor volume regression and tissue distribution of TMC. Type and ratio of used poloxamers were manipulated to provide the optimal gelation temperature (34e37 C). Rheological analysis showed low viscosity and elasticity values at low and room temperature while these values significantly increased at the physiological temperature. A prolonged diffusion-driven release of TMC was detected. In vivo data showed, evidently, that anticancer activity was improved with significant retention of the drug at the tumor site. These encouraging results confined that this in situ hydrogel depot offers an attractive approach for controlled delivery of TMC and clinically expected to be useful delivery system in loco-regional therapy for breast cancer.

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Cellular uptake, cytotoxicity and in-vivo evaluation of Tamoxifen citrate loaded niosomes

Dalia Samuel Shaker Kirolos

Dalia S. Shaker, Mohamed A. Shaker, Mahmoud S. Hanafy.

Int J Pharm

2015

One of the main challenges in Tamoxifen cancer therapy is achieving localized, efficient and sustained delivery without harming normal healthy organs. This study focused on evaluating Tamoxifen Citrate (TMC) niosomes for localized cancer therapy through in-vitro breast cancer cytotoxicity as well as in-vivo solid anti-tumor efficacy. Different niosomal formulae were prepared by film hydration technique and characterized for entrapment efficiency % (E. E), vesicle size, morphology, and in-vitro release. The cellular uptake and anti-cancer activity were also tested in-vitro using MCF-7 breast cancer cell line. Moreover, in-vivo anti-tumor efficacy was examined in Ehrlich carcinoma mice model through reporting solid tumor volume regression and tissue TMC distribution. The obtained niosomes prepared with Span 60: cholesterol (1: 1 molar ratio) showed a distinct nano-spherical shape with EE up to 92.3% ± 2.3. Remarkably prolonged release of TMC following diffusion release behavior was detected. The optimized formula showed significantly enhanced cellular uptake (2.8 fold) and exhibited significantly greater cytotoxic activity with MCF-7 breast cancer cell line. In-vivo experiment showed enhanced tumor volume reduction of niosomal TMC when compared to free TMC. Based on these results, the prepared niosomes demonstrated to be promising as a nano-size delivery vehicle for localized and sustained TMC cancer therapy.

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Guar gum and hydroxy propyl methylcellulose compressed coated tablets for colonic drug delivery: in vitro and in vivo evaluation in healthy human volunteers

Dalia Samuel Shaker Kirolos

Drug Discovry & Therapeutics

2011

ABSTRACT: The objectives of the present study are to evaluate guar gum in combination with hydroxy propyl methylcellulose (HPMC) as compression coat for colonic delivery of prednisolone as well as improving the mechanical properties of the compressed coated tablets. The core tablets containing 5 mg prednisolone were compression coated with 125 mg of coating materials consisted of guar gum alone or mixtures of guar gum in combination with different ratios of HPMC. The compressed coated tablets were evaluated for their mechanical properties, in vitro drug release and in vivo performance in human volunteers. The compressed coated tablets with coats containing HPMC exhibited acceptable mechanical properties. In vitro drug release studies in pH 7.4 phosphate-buffered saline medium containing 2% (w/v) rat caecal content have shown that increase in concentration of HPMC in the prepared coats from 10% to 20% resulted in an increase in the release rate. However, further increase in HPMC concentration to constitute 30% caused a reduction in the release rate. Based on the drug release results, tablets coated with coat consisted of 80% guar gum and 20% HPMC were selected for in vivo evaluation. In vivo gamma scintigraphic study on human volunteers using technetium-99m-diethylenetriamine pentaacetic acid as a tracer was performed. The results showed that tablets remained intact in stomach and small intestine, however partial and complete release of the tracer occurred in the colon. In conclusion, guar gum in combination with HPMC would be successfully used as a carrier for drug delivery to the colon.

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Bioavailability and hypocholesterolemic effect of proniosomal simvastatin for transdermal delivery.

Dalia Samuel Shaker Kirolos

Int J Pharm Pharm Sci.

2013

ABSTRACT Objective: Simvastatin (SIM) existing oral formulations suffer from poor bioavailability (less than 5%) as a result of extensive first-pass effect as well as dissolution rate-limited in vivo absorption. In the present study, a proniosomal system was designed for SIM transdermal delivery. Methods: In vitro evaluation of proniosomal SIM was performed in different aspects; drug entrapment, vesicle size, zeta potential, vesicular morphology, in vitro release, skin permeation and stability. The optimized formula was assessed for transdermal permeation in rats and for hypocholesterolemic effect in hypercholesterolemic rats compared to oral SIM dispersion. Results: The proniosomal formula consisted of lecithin: Tween 20 in molar ratio of 1:9 exhibited significantly (P<0.05) lower vesicular size, high SIM entrapment, sustained release pattern as well as significantly higher skin permeation. The topical application of optimized proniosomal SIM showed significantly (P<0.05) higher values of AUC0–8 and Tmax, and significantly (P<0.05) lower values of Cmax compared to SIM oral dispersion. The mean relative bioavailability of proniosomal SIM to oral dispersion was 120.40 ± 11.44%. The investigated proniosomal SIM showed a significantly (P<0.05) higher hypocholesterolemic effect compared to oral SIM dispersion in treatment of hypercholesterolemic rats. Conclusion: The obtained results were very encouraging and offered an alternative approach to enhance the bioavailability and the hypocholesterolemic effect of SIM. Keywords: Proniosomes, Simvastatin, Bioavailability, Hypocholesterolemic effect, Transdermal delivery.

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Mechanistic study of chemical permeation enhancers with different polar and lipophilic functional groups

Dalia Samuel Shaker Kirolos

Ning He, Kevin S. Warner, Doungdaw Chantasart, Dalia S. Shaker, William I. Higuchi, S. Kevin Li.

Journal of Pharmaceutical Sciences,

2004

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