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Staff Researches

Wafaa Moustafa El Kady

Comparative DNA profiling, botanical identification and biological evaluation of Gazania longiscapa DC and Gazania rigens L.

Wafaa Mostafa Hussain Ali Elkady

Wafaa M. El Kady, Abeer A.A. Salama , Samar Y. Desoukey,Eman G. Hagag, Siham M. El-Shenawy, M.A. El-Shanawany

Bulletin of Faculty of Pharmacy, Cairo University


Abstract Gazania longiscapa DC and Gazania rigens L. are species of cultivated ornamental plant that grow in Egypt. Genus Gazania has a role in folk medicine to prevent toothache; this study presents a comparative investigation of genetic and botanical features of root, rhizome, leaves and flowers of the two Gazania species and comparing their biological activity as analgesic and antiinflammatory as related to their folk medicinal use. The genetic and botanical differences between the two Gazania species are reported for the first time in this study. The results contribute toward validation of the traditional use of Gazania showing that both species are safe for oral administration and they exhibit significant antinociceptive and anti-inflammatory effects in a dose dependent manner.

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Hepatoprotection and Antioxidant Activity of Gazania longiscapa and G. rigens with the Isolation and Quantitative Analysis of Bioactive Metabolites

Wafaa Mostafa Hussain Ali Elkady

Samar Y Desoukey, Wafaa M El Kady, Abeer A A Salama, Eman G Hagag, Siham M El-Shenawy, M A El-Shanawany

International Journal of Pharmacognosy and Phytochemical Research 2016


Gazania longiscapa and G. rigens are two species belonging to family Asteraceae. The present study aimed the isolation of the main active constituents from the methanol extracts using different chromatographic methods and their identification using different spectroscopic techniques, beside the quantitation of some biologically important active constituent as rutin using HPLC technique, together with estimation of total polyphenolic content calculated as gallic acid and estimation of total flavonoid content calculated as rutin using UV technique. Concomitantly the determination of the antioxidant and hepatoprotective activity of the total methanol extracts of the aerial parts of G. longiscapa and G. rigens. This work resulted in the isolation of 4 flavonoids (Apigenin, Luteolin, Luteolin 7-O-β-D-glucopyranosid, Apigenin 7-O-β-D-glucopyranosid), 3 phenolic acids (Caffeic acid, Chlorogenic acid and 3,5- di- O-caffeoylquinic acid) from G. longiscapa for the first time; these 3 phenolic acids were also isolated from G. rigens, together with one flavonoid (rutin), The quantitative determination of the methanol extracts showed that G. longiscapa is a richer source of phenolic acids than G. rigens and both Gazania species are valuable sources of rutin beside having hepatoprotective and antioxidant activity.

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Polyphenolic Profile And Biological Activity Of Salvia Splendens Leaves

Wafaa Mostafa Hussain Ali Elkady

Fatma Abd-elkader Moharram, Mohamed Soubhi Marzouk, Siham Mustafa El-Shenawy and Wafaa Mostafa El Kady

European Journal Of Medicinal Chemistry 2012 Journal Of Pharmacy And Pharmacology 2012


Objectives: The aim of this study was to investigate a new flavone triglycoside, together with eleven phenolic metabolites from 80% aqueous methanol extract of S. Splendens leaves (AME) and assessment of its hypoglycemic and anti-inflammatory activities along with in vitro antioxidant effect. Methods: The phenolic composition of S. Splendens leaves was analyzed using UV, 1D and 2D NMR and negative ESI-MS spectroscopy. Hypoglycemic activity of AME was assessed by measuring blood glucose in streptozotocin induced diabetic rats. Key findings: Twelve phenolic metabolites including three phenolic acids, namely cafeeic acid 1, rosmarinic acid 2 and methyl rosmarinate 3, four flavone glycosides viz the new compound luteolin. Moreover, AME exhibited a significant anti-inflammatory activity only at 100 mg/kg in comparison to indomethacin. Finally, AME exhibited a marked significant scavenging activity against DPPPH, the maximum reactive reaction rate after 5 min was 62.9, 82.5, 83.7, 84.3 and 85.1 % for the concentration 10, 20, 30, 40 and 50 mg/ml, respectively. Conclusions: This is the first study reporting the identification of a new flavone along with eleven known phenolic metabolites from AME of S. Splendens. It showed significant hypoglycemic and anti-inflammatory effects in dose dependent manner. Moreover, it showed an in vitro antioxidant activity.

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