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Abdelfattah Ahmed Abdelkhalek Ahmed Soliman

Basic information

Name : Abdelfattah Ahmed Abdelkhalek Ahmed Soliman
Title: Lecturer
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Personal Info: Abdelfattah Ahmed, Lecturer at Department of Microbiology of Supplementary General Science, Faculty of Oral and Dental Medicine, Future University in Egypt, Cairo, Egypt

Education

Certificate Major University Year
PhD Microbiology Alazhahr University-Faculty of Scinces 2014
ماجستير Microbiology Faculty of Scinces - Alazhahr University 2010
بكالوريوس علوم 2005

Teaching Experience

Name of Organization Position From Date To Date
general science department teaching 01/01/2016 01/01/2018

Researches /Publications

Etoricoxib-loaded bio-adhesive hybridized polylactic acid-based nanoparticles as an intra-articular injection for the treatment of osteoarthritis - 01/0

Abdelfattah Ahmed Abdelkhalek Ahmed Soliman

Alaa H.Salama, Nermeen A.Elkasabgy

01/03/2020

Osteoarthritis is a major problem in elder people. Etoricoxib-loaded bio-adhesive hybridized nanoparticles were prepared using polylactic acid (PLA) and chitosan hydrochloride (CS-HCl) in presence of Captex®200 as a liquid oil, polyvinyl alcohol (PVA) and Tween®80 as surfactants. The study aimed to present a new intra-articular treatment of osteoarthritis with anti-inflammatory as well as bone rebuilding effects. Hybridized nanoparticles were fabricated applying the emulsion solvent evaporation technique then assessed for particle size, zeta potential, entrapment efficiency and in-vitro drug release. Furthermore, FT-IR and DSC in addition to morphological examination were done. Results revealed that the formulation composed of PLA:Captex®200 in ratio 1:2 (w/w), 1%w/v Tween®80, 0.3% w/v CS-HCl and 3%w/v PVA possessed the smallest particle size and the most sustained drug release, thus was sorted for further analyses. The selected formulation ability to interact with the negatively charged sodium fluroscein was evaluated to predict its binding with the naturally occurring hyaluronic acid in the knee joint where promising results were obtained. Results showed the cytocompatibility of the formulation when tested using MC3T3-E1 normal bone cell line, enhanced ALP activity and increased calcium ion deposition and binding. Results suggested that the presented formulation can be considered as an innovative approach for osteoarthritis.

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Bioactive injectable triple acting thermosensitive hydrogel enriched with nano-hydroxyapatite for bone regeneration: in-vitro characterization, Saos-2 cell line cell viability and osteogenic markers evaluation. - 01/0

Abdelfattah Ahmed Abdelkhalek Ahmed Soliman

Nadia M. Morsi,Rehab Nabil Shamma,Nouran Osama Eladawy

01/02/2019

Hydrogels forming in-situ have gained great attention in the area of bone tissue engineering recently, they were also showed to be a good and less invasive alternative to surgically applied ones. The primal focus of this study was to prepare chitosan-glycerol phosphate thermosensitive hydrogel formed in-situ and loaded with risedronate (bone resorption inhibitor) in an easy way with no requirement of complicated processes or large number of equipment. Then we investigated its effectiveness for bone regeneration. In-situ forming hydrogels were prepared using chitosan cross-linked with glycerol phosphate and loaded with risedronate and nano-hydroxyapatite as bone cement. The prepared hydrogels were characterized by analyzing their gelation time at 37 °C, % porosity, swelling index, in-vitro degradation, rheological properties, and in-vitro drug release. Results showed that the in-situ hydrogels prepared using 2.5% (w/v) chitosan cross-linked with 50% (w/v) glycerol phosphate in the ratio (9:1, v/v) reinforced with 20 mg/mL and nano-hydroxyapatite possessed the most sustained drug release profile. This optimized formulation was further evaluated using DSC and FTIR studies, in addition to their morphological properties using scanning electron microscopy. The effect on Saos-2 cell line viability was evaluated also using MTT assay on the optimized hydrogel formulation in addition to their action on cell proliferation using fluorescence microscope. Moreover, calcium deposition on the hydrogel and alkaline phosphatase activity were evaluated. Risedronate-nano-hydroxyapatite loaded hydrogels significantly enhanced the Saos-2 cell proliferation in addition to enhanced alkaline phosphatase activity and calcium deposition. Such results suggest that risedronate-nano-hydroxyapatite loaded hydrogels present great biocompatibility for bone regeneration. Proliferation of cells, as well as deposition of mineral on the hydrogel, was an evidence of the biocompatible nature of the hydrogel. This hydrogel formed in-situ present a good less invasive alternative for bone tissue engineering.

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Risedronate-Loaded Macroporous Gel Foam Enriched with Nanohydroxyapatite: Preparation, Characterization, and Osteogenic Activity Evaluation Using Saos-2 Cells - 01/0

Abdelfattah Ahmed Abdelkhalek Ahmed Soliman

Nadia M. Morsi, Rehab Nabil Shamma, Nouran Osama Eladawy

01/02/2019

The application of minimally invasive surgical techniques in the field of orthopedic surgery has created a growing need for new injectable synthetic materials that can be used for bone grafting. In this work, novel injectable thermosensitive foam was developed by mixing nHAP powder with a thermosensitive polymer with foaming power (Pluronic F-127) and loaded with a water-soluble bisphosphonate drug (risedronate) to promote osteogenesis. The foam was able to retain the porous structure after injection and set through temperature change of PF-127 solution to form gel inside the body. The effect of different formulation parameters on the gelation time, porosity, foamability, injectability, and in vitro degradation in addition to drug release from the prepared foams were analyzed using a full factorial design. The addition of a co-polymer like methylcellulose or sodium alginate into the foam was also studied. Results showed that the prepared optimized thermosensitive foam was able to gel within 1 min at 37°C, and sustain the release of drug for 72 h. The optimized formulation was further tested for any interactions using DSC and IR, and revealed no interactions between the drug and the used excipients in the prepared foam. Furthermore, the ability of the pre-set foam to support osteoblastic-like Saos-2-cell proliferation and differentiation was assessed, and revealed superior function on promoting cellular proliferation as confirmed by fluorescence microscope compared to the plain drug solution. The activity of the foam treated cells was also assessed by measuring the alkaline phosphatase activity and calcium deposition, and confirmed that the cellular activity was greatly enhanced in foam treated cells compared to those treated with the plain drug solution only. The obtained results show that the prepared risedronate-loaded thermosensitive foam would represent a step forward in the design of new materials for minimally invasive bone regeneration.

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Mesenchymal stem cells associated with chitosan scaffolds loaded with rosuvastatin to improve wound healing. - 01/0

Abdelfattah Ahmed Abdelkhalek Ahmed Soliman

Amr Maged, Azza A Mahmoud, Salwa Salah, Mahmoud M Ghorab

01/01/2019

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Design and characterization of emulsified spray dried alginate microparticles as a carrier for the dually acting drug roflumilast. - 01/0

Abdelfattah Ahmed Abdelkhalek Ahmed Soliman

Nermeen A.Elkasabgy

01/09/2018

Roflumilast is a selective inhibitor of phosphodiesterase-4 isoenzyme in lung cells. Having psychiatric adverse reactions when administered orally affects negatively the patients' adherence to the drug. This work aimed to prepare emulsified spray dried alginate microparticles for the pulmonary delivery of roflumilast. Sodium alginate was used as microparticle-forming material, isopropyl myristate as an oil, Tween®80 as surfactant and calcium beta-glycerophosphate as cross-linking agent to enhance the mechanical properties of the particles. The prepared particles were evaluated for their encapsulation efficiency, particle size and in-vitro drug release. From the studied carriers, beta-cyclodextrin (CD) was the best regarding giving formulation with smaller particle size and more sustained drug release. The inhalation profile of CD-based microparticles was investigated using Anderson cascade impactor. The aerosolization profile of CD-based microparticles suggested their efficiency to deliver the drug deep in the lung. The CD-based microparticles possessed more inhibitory effects on the viability of A549 cells and on the pro-inflammatory cytokines (TNF-α, IL-6 and IL-10) compared to the pure drug. Hence, CD-based microparticles could regulate the tumorigenesis besides tumor-associated inflammation. Finally, CD-based microparticles showed more sustained bronchodilatation properties in healthy human volunteers when compared to Ventolin®HFA. CD-based microparticles proved to be a promising carrier for inhaled roflumilast in human.

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Effects of Moringa Oleifera Aqueous Leaf Extract on Submandibular Salivary Glands of Diabetic Albino Rats - 01/0

Abdelfattah Ahmed Abdelkhalek Ahmed Soliman

01/04/2018

Moringa Oleifera (MO), also known as the ‘drumstick tree,’ is recognized as a nutritious and cheap source of phytochemicals, that a have a prominent anti-oxidative effect. Salivary gland dysfunction has been frequently reported in diabetic patients. Diabetes is a chronic metabolic disease that has complications mainly resulting from persistent hyperglycemia. Aim of the study: To Assess the effect of MO aqueous leaf extract on blood glucose levels in diabetic albino rats and its effect on submandibular salivary glands of diabetic albino rats. Materials and methods: the study comprised three groups; control, diabetic and MO treated groups. The experiment was terminated after fourteen days. The evaluation was performed by measuring the blood glucose levels and weight. Histological evaluation was done by H&E, PAS and IHC for COX-2. Results: the blood glucose levels and histological signs of diabetic complications were significantly lowered in the MO treated group. Conclusion: MO is a promising anti-diabetic treatment and may even reverse some of the diabetic complications.

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Dihydrofolate reductase (DHFR) inhibition and molecular modeling study of some 6-bromo- or 6,8-dibromo-quinazolin-4(3H)-ones. - 01/0

Abdelfattah Ahmed Abdelkhalek Ahmed Soliman

01/04/2018

Objectives: The dihydrofolate reductase (DHFR) inhibitory activity of 6-bromo- and 6,8-dibromo-quinazolin-4(3H)-ones (7–25) were studied to define the structural features and requirements that enhance selectivity and specificity for the proper binding to the enzyme active site. Methods: Compounds 7–25 were tested for their in vitro DHFR inhibition. As an application of the use of DHFR inhibitors, in vitro antitumor activity using disease-oriented human cell lines assay was performed. Key findings: Compounds 19, 20, and 22 showed remarkable DHFR inhibitory activity, inhibitory concentration (IC50 0.6, 0.2, and 0.1 μM, respectively). Compounds 12, 17, 18, 20, and 24 proved to be broad spectrum antitumor with median IC50 values of 0.6, 0.6, 0.5, 0.6, and 0.7 μM, respectively. Molecular docking study results revealed that the active DHFR inhibitors 22 and 20 bind to DHFR with similar amino acid residues as methotrexate, especially Arg 28. Conclusions: The mono-bromo series proved to be more active than the di-bromo counterparts and the 3-(2-hydrazinyl-acetyl)- is more active than its 3-(acetohydrazide) isoster. The investigated compounds could be used as template model for further optimization.

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Derivatives of Cucurbitacin-E-Glucoside Produced by Curvularia lunata NRRL 2178: Anti-inflammatory, Antipyretic, Antitumor Activities, and Effect on Biochemical Parameters - 01/0

Abdelfattah Ahmed Abdelkhalek Ahmed Soliman

Abdel-Monem M.A.Sharaf, Mohamed Rabie, Hussein I.El-Subbagh

01/04/2017

E glucoside (1) is a tetracyclic triterpenoid glucoside, isolated from Cucurbitaceae plants. In the present study the pharmacological and biochemical effects of cucurbitacin-E-glucoside and two of its microbial transformation derivatives (2 and 3) produced by Curvularia lunata NRRL 2178, were investigated. The isolated compounds were identified by 1H and 13C NMR spectroscopy. The obtained results showed that 2 and 3 possess anti-inflammatory and antipyretic effects, compared with indomethacin and acetaminophen. Oral administration of 2 and 3 at dose of 1/10 of LD50 for 30 days resulted in a significant decrease in the serum levels of glucose, cholesterol and triacylglycerol. Cucurbitacin–E-glucoside showed moderate cytotoxicity against tumor cell lines while compound 3 proved to be selective against colon carcinoma cell lines.

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