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Staff Researches

Wafaa Moustafa El Kady

Difference in chemical composition and antimicrobial activity of Thymus capitatus L. essential oil at different altitudes

Wafaa Mostafa Hussain Ali Elkady

Lamya F.A. El-Jalel , Mariam H. Gonaid , Kamel A. El-Gareeb

Future Journal of Pharmaceutical Sciences

2018

The aim of the present study is to investigate the effect of different altitude on the chemical composition and antimicrobial activity of the essential oil prepared from wild Libyan growing Thymus capitatus L. aerial parts collected from Al-Jabal Akhdar. Two different altitudes were chosen represented by Abu-Draa (650 m above the see level) and Sidi Al-Hamey (850 m above the see level). This study concerned with both qualitative and quantitative variations. The percentage yield for T. capitatus essential oils yield were 1.5% and 1.04% for both Abu-Draa and Sidi Al-Hamry respectively. GC/MS analysis for each essential oil revealed the identification of 14 volatile components corresponding to 91.99% from Abu-Draa and 23 compounds corresponding to 96.54% from Sidi Al-Hamrey. Carvcrol was the major volatile component present in both essential oils of Abu-Draa and Sidi Al-Hamrey regions (58.56% and 24.28% respectively). The second major volatile compound present in the essential oil prepared from Abu-Draa is b-caryophyllene (7.41%) followed by its oxide (6.26%); on the other hand Gamma-terpinene is the second major volatile compound present in Sidi Alhamry prepared oil (16.18%) followed by caryophyllene oxide (10.43%). Both phenolic and phenolic ethers are dominated in both examined oils (62.51, 33.74% respectively) resulting in a great antimicrobial activity for both of them which is more prominent in that oil prepared from the low altitude Abu-draa.

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Chemical Profile and Biological Activity of Casimiroa Edulis Non-Edible Fruit`s Parts

Wafaa Mostafa Hussain Ali Elkady

Eman Ahmed Ibrahim , Mariam Hussein Gonaid , Farouk Kamel El Baz

Advanced Pharmaceutical Bulletin

2017

Purpose: the non-edible fruit parts of Casimiroa edulis Llave et were evaluated for their active constituents and their potential as antioxidants, anti-inflammatory and antitumor activity. Methods: Fruits peel (FP) and seeds kernel (SK) of Casimiroa edulis Llave et Lex. were extracted successively with hexane and then methanol. Fatty acids were prepared from hexane extracts and identified by GC. Total flavonoid, phenolic acids and tannins contents in methanol extracts were determined by UV spectrophotometer and identified by HPLC. Antioxidant, invitro anti-inflammatory activity and antitumor effect against Caco-2 cell line were determined . Results: GC analysis of hexane extracts showed that oleic acid (47.00%) was the major unsaturated fatty acids in both extracts while lignoceric acid (15.49%) is the most abundant saturated fatty acid in (FP). Total phenolic, flavonoid and tannin contents in (FP) & (SK) methanol extracts were; 37.5±1.5, 10.79±0.66 and 22.28±0.23 for (FP); 53.5±1.5mg/g, 14.44±0.32 mg/g; and 53.73±3.58 mg/g for (SK) respectively. HPLC analysis of methanol extract revealed that; the major phenolic compound was pyrogallol in (FP) and phydroxybenzoic acid in (SK), the major flavonoid was luteolin 6-arabinose-8-glucose in (FP) and acacetin in (SK). Conclusion: This study showed that non-edible parts of C. edulis fruit is a rich source of different phenolic compounds and fatty acids which has great antioxidant, anti-inflammatory and antitumor activities; that could be used as a natural source in pharmaceutical industry.

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Comparative DNA profiling, botanical identification and biological evaluation of Gazania longiscapa DC and Gazania rigens L.

Wafaa Mostafa Hussain Ali Elkady

Wafaa M. El Kady, Abeer A.A. Salama , Samar Y. Desoukey,Eman G. Hagag, Siham M. El-Shenawy, M.A. El-Shanawany

Bulletin of Faculty of Pharmacy, Cairo University

2015

Abstract Gazania longiscapa DC and Gazania rigens L. are species of cultivated ornamental plant that grow in Egypt. Genus Gazania has a role in folk medicine to prevent toothache; this study presents a comparative investigation of genetic and botanical features of root, rhizome, leaves and flowers of the two Gazania species and comparing their biological activity as analgesic and antiinflammatory as related to their folk medicinal use. The genetic and botanical differences between the two Gazania species are reported for the first time in this study. The results contribute toward validation of the traditional use of Gazania showing that both species are safe for oral administration and they exhibit significant antinociceptive and anti-inflammatory effects in a dose dependent manner.

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Hepatoprotection and Antioxidant Activity of Gazania longiscapa and G. rigens with the Isolation and Quantitative Analysis of Bioactive Metabolites

Wafaa Mostafa Hussain Ali Elkady

Samar Y Desoukey, Wafaa M El Kady, Abeer A A Salama, Eman G Hagag, Siham M El-Shenawy, M A El-Shanawany

International Journal of Pharmacognosy and Phytochemical Research 2016

2016

Gazania longiscapa and G. rigens are two species belonging to family Asteraceae. The present study aimed the isolation of the main active constituents from the methanol extracts using different chromatographic methods and their identification using different spectroscopic techniques, beside the quantitation of some biologically important active constituent as rutin using HPLC technique, together with estimation of total polyphenolic content calculated as gallic acid and estimation of total flavonoid content calculated as rutin using UV technique. Concomitantly the determination of the antioxidant and hepatoprotective activity of the total methanol extracts of the aerial parts of G. longiscapa and G. rigens. This work resulted in the isolation of 4 flavonoids (Apigenin, Luteolin, Luteolin 7-O-β-D-glucopyranosid, Apigenin 7-O-β-D-glucopyranosid), 3 phenolic acids (Caffeic acid, Chlorogenic acid and 3,5- di- O-caffeoylquinic acid) from G. longiscapa for the first time; these 3 phenolic acids were also isolated from G. rigens, together with one flavonoid (rutin), The quantitative determination of the methanol extracts showed that G. longiscapa is a richer source of phenolic acids than G. rigens and both Gazania species are valuable sources of rutin beside having hepatoprotective and antioxidant activity.

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DNA with botanical profiling and biological study of 2 Gazania species

Wafaa Mostafa Hussain Ali Elkady

LAP LAMBERT Academic Publishing

2016

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Chemical profiling and antiproliferative effect of essential oils of two Araucaria species cultivated in Egypt

Wafaa Mostafa Hussain Ali Elkady

Iriny M. Ayoub

Industrial Crops & Products

2018

Araucaria is a small genus of evergreen coniferous trees, widely used for ornamental and timber purposes. Araucaria species are rich in essential oils. In this study, the essential oils obtained from the foliage of Araucaria heterophylla (Salisb.) and Araucaria bidwillii Hook were analyzed using GC and GC/MS. Cytotoxicity of both essential oils was assessed on three human cancer cell lines using MTT assay. Twenty nine compounds were annotated in A. heterophylla essential oil, whereas forty three were identified in A. bidwillii accounting for 95.16% and 81.06% of the total volatile oil composition, respectively. α-Pinene (70.85%), D-limonene (4.26%) and germacrene D (2.99%) represented the major compounds in A. heterophylla. Beyerene (35.65%), transnerolidol (13.66%) and ɣ-elemene (6.09%) dominated in A. bidwillii. Both oils showed potent cytotoxicity against all tested cancer cell lines (Hep-G2, MCF-7 and Caco-2) exhibiting IC50 values of 0.70, 3.20 and 1.10 μg/ ml for A. heterophylla; and 1.67, 1.10 and 1.32 μg/ml for A. bidwillii, respectively. Caspase-3 activation indicated that the cytotoxicity of A. bidwillii essential oil is mediated via caspase-dependent apoptosis. These findings suggest that the essential oils of A. heterophylla and A. bidwilli offer promising anticancer drug candidates pertaining to the synergistic effects of their individual components.

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Polyphenolic Profile And Biological Activity Of Salvia Splendens Leaves

Wafaa Mostafa Hussain Ali Elkady

Fatma Abd-elkader Moharram, Mohamed Soubhi Marzouk, Siham Mustafa El-Shenawy and Wafaa Mostafa El Kady

The Journal of pharmacy and pharmacology

2012

Objectives: The aim of this study was to investigate a new flavone triglycoside, together with eleven phenolic metabolites from 80% aqueous methanol extract of S. Splendens leaves (AME) and assessment of its hypoglycemic and anti-inflammatory activities along with in vitro antioxidant effect. Methods: The phenolic composition of S. Splendens leaves was analyzed using UV, 1D and 2D NMR and negative ESI-MS spectroscopy. Hypoglycemic activity of AME was assessed by measuring blood glucose in streptozotocin induced diabetic rats. Key findings: Twelve phenolic metabolites including three phenolic acids, namely cafeeic acid 1, rosmarinic acid 2 and methyl rosmarinate 3, four flavone glycosides viz the new compound luteolin. Moreover, AME exhibited a significant anti-inflammatory activity only at 100 mg/kg in comparison to indomethacin. Finally, AME exhibited a marked significant scavenging activity against DPPPH, the maximum reactive reaction rate after 5 min was 62.9, 82.5, 83.7, 84.3 and 85.1 % for the concentration 10, 20, 30, 40 and 50 mg/ml, respectively. Conclusions: This is the first study reporting the identification of a new flavone along with eleven known phenolic metabolites from AME of S. Splendens. It showed significant hypoglycemic and anti-inflammatory effects in dose dependent manner. Moreover, it showed an in vitro antioxidant activity.

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