Author : Mohamed Mohamed
CoAuthors : Mohamed A. R. Korashy , Ahmed S. Saad, Sherif A. Abdel Gawad , , Medhat A. Al-Ghobashy, Nagiba Y. Hassan
Source : Current Science International
Date of Publication : 07/2018
Abstract :
Development of analytical methods capable of selective determination and quantification of
chemical compounds in industrial wastewater have become essential as these compounds lead to
alteration of the ecosystem causing serious effects on public health; thereby, development of a sensitive
and selective method for the simultaneous determination of four commonly used non-steroidal antiinflammatory
drugs, namely; paracetamol, diclofenac sodium, ibuprofen and indomethacin in
wastewater. Solid phase extraction (SPE) was carried out for the selective extraction and
preconcentration of the drugs from the water samples. Multivariate calibration of spectrophotometric
absorbance data using Partial Least Squares (PLS) regression analysis. As per the multilevel multifactor
design, calibration and validation sets were fabricated and the model was constructed using mean
centered data of the calibration set, leaving out one mixture at a time for cross-validation, then, the
method was applied blindly for determination of the four drugs in the validation set. The method was
validated according to the ICH guidelines, and the mentioned chemometric method were successfully
applied for the selective determination of the four mentioned drugs in real wastewater samples. Prior to
the application of chemometric technique the actual wastewater samples were subjected to SPE for
pretreatment and preconcentration.
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