Future University In Egypt (FUE)
Future University is one of most promising private universities in Egypt. Through excellence in teaching, research and service, Future University strives to provide a comprehensive, high-quality education that prepares our graduates to be future leaders.
mainLogo
90th Street
New Cairo
Egypt

Amr Mohamed Badawy

Basic information

Name : Amr Mohamed Badawy
Title: Professor of Analytical Chemistry
Google Schoolar Link
Personal Info: Professor Amr Badawy, Professor of Analytical Chemistry - Department of Pharmaceutical Chemistry. He has a Ph.D and a MSC degree in Analytical Chemistry from Cairo university. View More...

Education

Certificate Major University Year
PhD Analytical Chemistry Cairo University -Faculty of Pharmacy 2003
Masters Analytical Chemistry Faculty of Pharmacy, Cairo University 1996
Bachelor Analytical Chemistry Faculty of Pharmacy, Cairo University 1991

Teaching Experience

Name of Organization Position From Date To Date
Faculty of Pharmacy, Cairo University Assistant Professor 01/01/2008 01/01/2013
Faculty of Pharmacy, Cairo University Lecturer 01/01/2003 01/01/2008
Faculty of Pharmacy, Cairo University Assistant Lecturer 01/01/1996 01/01/2003
Faculty of Pharmacy, Cairo University Demonstrator 01/01/1991 01/01/1996

Researches /Publications

Spectral resolution of quaternary components in a sinus and congestion mixture; Multivariate algorithms to approach extremes of concentration levels. - 01/0

Amr Mohamed Mohamed Badawey

Yehia AM, Nabil M, Abbas SS.

01/05/2020

Download PDF
Analytical methods for the determination of paracetamol, pseudoephedrine and brompheniramine in Comtrex® tablets - 01/0

Amr Mohamed Mohamed Badawey

Souha Hosam Youssef , Dalia Mohamed, Maha Abdel Monem Hegazy

01/05/2019

Comtrex® tablets composed of paracetamol, pseudoephedrine and brompheniramine are widely used for relieving symptoms related to common cold. This study has overcome the challenging dosage form ratio (250:15:1) and proposed chromatographic methods for analyzing the ternary combination were utilized displaying different apparatus, solvents and sensitivity ranges. Three chromatographic methods namely thin layer chromatography (TLC), high performance liquid chromatography with ultra-violet detection (HPLC–UV) and ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) were developed and validated for the simultaneous determination of the three drugs. Concerning the TLC method, aluminum TLC plates pre-coated with silica gel 60F254 were used and methanol:water:ammonia (9:1:0.1, v/v/v) was applied as a mobile phase; scanning of the plates was carried out at 254 nm. For the HPLC–UV method C18 column was used with an isocratic elution mobile phase composed of water:acetonitrile (75:25, v/v; pH 3.2) and the detection was at 210 nm. For the UPLC-MS/MS method; separation was performed on a UPLC-BEH C18 column with methanol: 0.1% ammonium formate (60:40, v/v) as the mobile phase utilizing diphenhydramine as an internal standard and mass spectrometry was used for detection. The methods were simple, sensitive, accurate and precise. Statistical analysis revealed no significant difference from the reported methods in regard to accuracy and precision

Download PDF
Spectrophotometric resolution and quantification of ternary co-formulated mixture of thioctic acid, benfotiamine and cyanocobalamin - 01/0

Amr Mohamed Mohamed Badawey

Hegazy Maha, Abbas Samah

01/04/2019

Four spectrophotometric methods were developed and validated for spectral resolution and determination of thioctic acid (THC), benfotiamine (BEN) and cyanocobalamin (CNCo) in their pure form, laboratory prepared mixtures and capsules. Method A, a first derivative method for determination of CNCo, by recording the peak amplitude at 564.0 nm. Method B, is a Ratio difference spectrophotometric one which is applied for determination of THC and BEN, where CNCo has no interference. Method C applied first derivative of ratio spectra where THC and BEN were determined by recording the peak amplitude at 330.8 and 286.8 nm, in order. Method D a Ratio subtraction method that was successfully determines BEN at its λmax without interference of the other co-formulated drugs. Validation according to ICH guidelines was performed. The linearity ranges were 200.00–1800.00, 2.00–30.00 and 10.00–200.00 μg/mL for THC, BEN and CNCo, in a respective order. The methods were found to be accurate, precise and specific.

Download PDF
Analysis of paracetamol, pseudoephedrine and cetirizine in Allercet Cold® capsules using spectrophotometric techniques - 01/0

Amr Mohamed Mohamed Badawey

Souha H. Youssef, Maha Abdel-Monem Hegazy, Dalia Mohamed

01/06/2018

Paracetamol (PAR), Pseudoephedrine hydrochloride (PSE) and cetirizine dihydrochloride (CET) is a ternary mixture that composes tablets which are popular for the relief of flu in Egypt. The spectra of the drugs were overlapped and no spectrophotometric methods were reported to resolve the mixture. This research proposes four spectrophotometric methods that are efficient and require water only as a solvent. The first method was ratio subtraction-ratio difference method (RSDM) where PAR was initially removed from the mixture by ratio subtraction and determined at 292.4 nm, then PSE and CET were quantified by subtracting the amplitudes of their ratio spectra between 257.0 and 230.0 nm for PSE and between 228.0 and 257.0 nm for CET. The second method was derivative ratio spectra—zero crossing (DRZC) which was based on determining both PSE and CET from the zero-crossing points of the first and third derivative of their ratio spectra at 252.0 and 237.0 nm, respectively while PAR was determined using its first derivative at 292.4 nm. Moreover, the ternary mixture was resolved using successive derivative ratio (SDR) method where PAR, PSE and CET were determined at 310.2, 257.0 and 242.4 nm, respectively. The fourth proposed method was pure component contribution algorithm (PCCA) which was applied to quantify the drugs at their λmax. Recovery percentages for RSDM were 100.7 ± 1.890, 99.69 ± 0.8400 and 99.38 ± 1.550; DRZC were 101.8 ± 0.8600, 99.04 ± 1.200 and 98.95 ± 1.300; SDR were 101.9 ± 1.060, 99.59 ± 1.010 and 100.2 ± 0.6300; PCCA were 101.6 ± 1.240, 99.10 ± 0.5400 and 100.4 ± 1.800 for PAR, PSE and BRM; respectively. The suggested methods were effectively applied to analyze laboratory prepared mixtures and their combined dosage form.

Download PDF
Experimental validation of a computationally designed tiotropium membrane sensor - 01/0

Amr Mohamed Mohamed Badawey

Ali M. Yehia , Soad E. Abo-Elhoda , Nagiba Y. Hassan

01/06/2018

AbstractIn this study we draw parallel computational and experimental data for potentiometric sensor assembly. Tiotropium (TIO) is a long acting anticholinergic agent and is used for maintenance treatment of chronic obstructive pulmonary disease. Systems comprising different ion exchangers with or without ionophores were theoretically and practically evaluated for TIO membrane sensors. Different phenyl borate derivatives as cation exchangers along with calix[8]arene or (2-hydroxypropyl)-β-cyclodextrin as ionophores were compared in terms of steric energies and detection limits. Tetrakis-[3,5-bis(trifluoro-methyl)phenyl] borate and calix[8]arene sensors provided the lowermost steric energy (−30.63 kcal mol−1) as well as detection limit (1.6 × 10−7 mol L−1) for TIO analysis. Correlation between the normalized values of theoretical energies and practical detection limits was reasonably close; therefore, molecular mechanics offers a viable alternative to experimental optimization of sensor assembly. The optimized sensor showed a Nernstian slope of 59.5 mV decade−1 in a linear range from 3.2 × 10−7 mol L−1 to 1 × 10−2 mol L−1. This sensor could sufficiently discriminate the target ion from N-methyl-scopine as a possibly interfering official impurity. Selectivity was enhanced by about one order of magnitude compared to other sensors. The proposed sensor was successfully used for the determination of TIO in Spiriva® inhalation powder and compared favourably with the official method.

Download PDF
Analysis of paracetamol, pseudo-ephedrine and brompheniramine in comtrex tablets using chemometric methods. - 01/0

Amr Mohamed Mohamed Badawey

Souha Hosam Youssef, Maha Abdel Moneim Hegazy, Dalia Mohame

01/05/2017

Paracetamol (PAR), pseudoephedrine hydrochloride (PSE) and brompheniramine maleate (BRM) are co-formulated drugs that are widely used in the Egyptian market for the relief of symptoms associated with common cold. Their severely overlapped spectra were resolved by two simple, accurate and precise chemometric techniques, principal component regression method (PCR) and partial Least Squares methods (PLS). The proposed methods were rapid, cost effective and were successfully applied for the analysis of laboratory prepared mixtures and the combined dosage form. Good recoveries were obtained for PCR method, 100.42, 100.05 and 98.96 % and for PLS method 100.04, 99.95 and 100.36 % for PAR, PSE and BRM, respectively. The methods were validated according to the ICH guidelines. Comparison of the applied methods with the reported method was done and no significant difference was found regarding accuracy and precision.

Download PDF
Selective determination of tolterodine tartrate in presence of its oxidative - 01/1

Amr Mohamed Mohamed Badawey

Mamdouh R.Rezk1*,AmrM.Badawy1,2, OsamaAbdel Sattar3, OmaimaM.Khattab4

01/10/2016

Four stability-indicating methods were developed for determination of tolterodine tartrate in the presence of its oxidative degradation product (the metabolite). The degradation product was prepared via oxidation with hydrogen peroxide. The degradation product was characterized and structurally elucidated. The first method was the first derivative 1D by measuring the peak amplitude at 292nm. The second method was a second derivative by measuring the peak amplitude at 236, 287, 296 nm. The third method was a high performance liquid chromatographic using a reversed phase column and a mobile phase of phosphate buffer: methanol: triethyl amine (60: 40; 0.2 by volume). The forth method was a thin layer chromatography coupled with densitometric detection. Selective quantification of tolterodine in pure form, pharmaceutical formulation and/or in the presence of its degradant was demonstrated. The indication of stability was done under condition likely to be expected at normal storage condition.

Download PDF
Determination of sparfloxacin and besifloxacin hydrochlorides using gold nanoparticles modified carbon paste electrode in micelliar medium - 01/0

Amr Mohamed Mohamed Badawey

Ali K. Attia,*a Amr M. Badawyb and Samr G. Abd-Elhamida

01/06/2016

A gold nanoparticles modified carbon paste electrode (AuCPE) was used to study the electrochemical behavior of sparfloxacin HCl (SPAR) and besifloxacin HCl (BESI) using cyclic and differential pulse voltammetry modes in the presence of micellar medium. Effect of different surfactants on peak current was studied in Britton–Robinson buffer solution of pH 2. Sodium dodecyl sulphate is the optimum surfactant based on the enhancement of the peak current. The modified electrode shows highly sensitive sensing giving an excellent response for SPAR and BESI. The peak current varied linearly over the concentration ranges from 1.1  107 mol L1 to 3.3  106 mol L1 and from 2.2  106 mol L1 to 5.5  105 mol L1 with determination coefficients of 0.9976 and 0.9984 in case of SPAR and BESI, respectively. The recoveries and the relative standard deviations were found in the following ranges: 99.97–101.4% and 0.63–1.48% for SPAR and 99.89–101.1% and 0.85–1.76% for BESI. The detections limits were 2.87  108 and 3.76  107 mol L1 for SPAR and BESI, respectively. The proposed method has been successfully applied to determine SPAR and BESI in biological fluids.

Download PDF
Determination of sparfloxacin and besifloxacin hydrochlorides using gold nanoparticles modified carbon paste electrode in micellar medium - 01/0

Amr Mohamed Mohamed Badawey

Ali K. Attia, Samr G. Abd-Elhamida

01/04/2016

A gold nanoparticles modified carbon paste electrode (AuCPE) was used to study the electrochemical behavior of sparfloxacin HCl (SPAR) and besifloxacin HCl (BESI) using cyclic and differential pulse voltammetry modes in the presence of micellar medium. Effect of different surfactants on peak current was studied in Britton–Robinson buffer solution of pH 2. Sodium dodecyl sulphate is the optimum surfactant based on the enhancement of the peak current. The modified electrode shows highly sensitive sensing giving an excellent response for SPAR and BESI. The peak current varied linearly over the concentration ranges from 1.1 × 10−7 mol L−1 to 3.3 × 10−6 mol L−1 and from 2.2 × 10−6 mol L−1 to 5.5 × 10−5 mol L−1 with determination coefficients of 0.9976 and 0.9984 in case of SPAR and BESI, respectively. The recoveries and the relative standard deviations were found in the following ranges: 99.97–101.4% and 0.63–1.48% for SPAR and 99.89–101.1% and 0.85–1.76% for BESI. The detections limits were 2.87 × 10−8 and 3.76 × 10−7 mol L−1 for SPAR and BESI, respectively. The proposed method has been successfully applied to determine SPAR and BESI in biological fluids.

Download PDF
Stability-Indicating Methods for the Determination of Gemifloxacin in Presence of Its Acid Degradation Product - 01/1

Amr Mohamed Mohamed Badawey

Ezzat M. Abdel-Moety, Amr M. Badawey, Hebatallah M. Essam and Fatma M. Aboul Alamine

01/10/2015

Brilliant, valid and simple five UV spectrophotometric stability indicating techniques are adopted for the determination of Gemifloxacin (GEM) in presence of its acid degradation products over a concentration range of 2-12 μg mL-1. The first method is an application of the first derivative (1D) spectrophotometry, that allows the determination of GEM without interference of its acid degradation products at zero crossing wavelength (254.6 nm). The second method depends on the first-derivative of the ratio spectra spectrophotometry (1DD) for determination of GEM in presence of its acid degradation products at a maximum of 273.0 nm and a minimum of 284.0 nm, While the third dual wavelength method offers a superior stability indicating procedures for the determination of GEM in the zero order spectra at the wavelength pair of 271.8 nm and 325.0 nm. The fourth method is the ratio difference one, with the advantages of minimal data processing and wide range of application. It is applied for the analysis of intact drug in presence of its acid degradation products by measuring the difference in the peak amplitude at the ratio spectra at 355.0 nm and 270.0 nm. The last method is based on the quantification of GEM through the bivariate calibration at 255.0 nm and 277.0 nm by adopting simple mathematic algorithm that provides simplicity and rapidity.

Download PDF
Analysis of Stiripentol Enantiomers on Several Chiral Stationary Phases: A Comparative Study - 01/0

Amr Mohamed Mohamed Badawey

Ola A. Saleh,Mohammed F. El-Behairy,Aida A. El-Azzouny,Hassan Y. Aboul-Enein

01/01/2015

A comparative study was developed for the separation of stiripentol enantiomers on several chiral stationary phases which were CyclobondI 2000®, S,S Whelk O1®, R,R Whelk O1®, Chiralcel OB®, Chiralcel OF®, Chiralcel OB-H® and Chiralpak AD-RH®. The best separation was achieved on Chiralpak AD-RH chiral column where a simple, rapid and validated method for the determination of stiripentol enantiomers was developed. Stiripentol was separated and quantitated on Chiralpak AD-RH chiral column using a mixture of water/acetonitrile (30/70 v/v) as a mobile phase (t 1, t 2 = 5.626, 6.891, α = 1.22, R s = 2.53) at 25 °C and a flow rate of 1 mL min−1. The UV-detector was set at 254 nm. The applied HPLC method allowed the separation and quantification of stiripentol enantiomers with good linearity (r > 0.999) in the studied range. The relative standard deviations (% RSD) were 0.723 and 0.692 for the stiripentol enantiomers with accuracy of 98.40 and 98.53. The limit of detection and limit of quantification of stiripentol were found to be 10 and 30 µg mL−1, respectively. The method was validated through the parameters of linearity, accuracy, precision and robustness. The HPLC method was applied for the quantitative determination of stiripentol in pharmaceutical formulations.

Download PDF
Electroanalytical Determination of Gemifloxacin Mesylate in Bulk, Tablets and - 01/1

Amr Mohamed Mohamed Badawey

A.K. Attiaa,, M.M. Abd-Elmoetya, A.M. Badawyb, A.E. Abd-Elaleemb and S.G. Abd-Elhamida

01/12/2014

A simple, precise, inexpensive and sensitive voltammetric method has been developed for the determination of gemifloxacin mesylate (GEM) in the presence of tween 80 in the bulk, farmaceutical dosage forms and human urine at gold nanoparticles modified carbon paste electrode (GNCPE). The electrochemical behavior of GEM has been investigated by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. The electrochemical oxidation of GEM was an irreversible process which exhibited adsorption-diffusion controlled process behavior in Britton-Robinson (BR) buffer over the entire pH range of values from 2 to 9. The adsorptive stripping response was evaluated as a function of some variables such as pH, type of surfactant, scan rate and accumulation time. The anodic peak current varied linearly over the range from 8.0 × 10-7 to 2.8 × 10-5 M. The limits of detection and quantification were 7.32 × 10-8 M and 2.44 × 10-7 M, respectively. The relative standard deviations and the percentage recoveries were found in the following ranges: 0.58-1.35% and 99.37-101.76%, respectively

Download PDF
A validated stability indicating HPLC method for determination of sitagliptin - 01/0

Amr Mohamed Mohamed Badawey

Ola Ahmed Saleh, Aida Abd El-Sattar El-Azzouny, Hassan Youssef Aboul-Enein

01/01/2014

A comparative and stability-indicating reversed phase high performance liquid chromatographic study have been developed and validated for sitagliptin phosphate. The liquid chromatographic determination was achieved isocratically on Poroshell 120 EC-C18 (100 × 4.6 mm, i.d.; particle size, 2.7 µm), Pursuit 5PFP (150 × 4.6 mm, i.d.; particle size, 5 µm) and Chromolith performance RP-18e (100 × 4.6 mm, i.d.; macropore diameter, 2 µm) columns using a mobile phase consisting of methanol:water:triethylamine:acetic acid (60:40:0.1:0.1; v:v:v:v), at a flow rate 0.5 mL/min and UV detection at 268 nm. The method was linear over the concentration range of 100-1000 µg/mL (r = 0.9998) with a limit of detection and quantitation of 10 and 30 µg/mL, respectively. All the validation parameters and stability indicating study were studied on Poroshell 120 EC-C18 column, which achieved the best separation. The proposed method has been found to have the required accuracy, selectivity, sensitivity, and precision to assay sitagliptin phosphate in bulk form and in a pharmaceutical dosage form. Degradation products resulting from the stress studies did not interfere with the detection of sitagliptin phosphate that indicates that the assay are stability-indicating assay.

Download PDF
6. Stability – Indicating Methods for the Determination of Erdosteine In the Presence of Its Acid Degradation Products; Nadia M. Moustafa - 01/0

Amr Mohamed Mohamed Badawey

Nadia M. Moustafa, Nesrine T. Lamie1, and Abd El-Aziz B. Abd El-Aleem

01/01/2013

Four accurate, sensitive, and reproducible stability-indicating methods for the determination of erdosteine in the presence of its acid degradation products are presented. The first method involves processing the spectra by using a first-derivative method at 229 nm in a concentration range of 10–70 μg/mL. The mean percentage recovery was 100.43 ± 0.977. The second method is based on ratio-spectra first derivative spectrophotometry at 227.4 and 255 nm over a concentration range of 10–70 μg/mL. The mean percentage recovery was 99.65 ± 1.122% and 100.02 ± 1.306% at 227.4 and 255 nm, respectively. The third method utilizes quantitative densitometric evaluation of the TLC of erdosteine in the presence of its acid degradation products, and uses methanol–chloroform–ammonia (7 + 3 + 0.01, v/v/v) as the mobile phase. TLC chromatograms were scanned at 235 nm. This method analyzes erdosteine in a concentration range of 2.4–5.6 μg/spot, with a mean percentage recovery of 100.03 ± 1.015%. The fourth method is HPLC for the simultaneous determination of erdosteine in the presence of its acid degradation products. The mobile phase consists of water–methanol (65 + 35, v/v). The standard curve of erdosteine showed good linearity over a concentration range of 10–80 μg/mL, with a mean percentage recovery of 99.90 ± 1.207%. These methods were successfully applied to the determination of erdosteine in bulk powder, laboratory-prepared mixtures containing different percentages of the degradation products, and pharmaceutical dosage forms. The validity of results was assessed by applying the standard addition technique. The results obtained agreed statistically with those obtained by a reported method, showing no significant differences with respect to accuracy and precision. Stability-Indicating Methods for the Determination of Erdosteine in the Presence of its Acid Degradation Products. Available from: https://www.researchgate.net/publication/261181729_Stability-Indicating_Methods_for_the_Determination_of_Erdosteine_in_the_Presence_of_its_Acid_Degradation_Products [accessed Dec 14, 2015].

Download PDF
10. Stability Indicating Methods for the Determination of Rosuvastatin Calcium in the Presence of its oxidative Degradation Products - 01/0

Amr Mohamed Mohamed Badawey

Nadia M Mostafa, Nesrine T Lamie* and Abd El-Aleem AEB

01/01/2012

Four different accurate, sensitive and reproducible stability-indicating methods for the determination of rosuvastatin calcium in the presence of its oxidative degradation products are presented. The first method is Second-derivative (2D) method at 243.6 nm in a concentration range of 5-30 μg mL−1 with mean percentage recovery of 99.94±1.171. The second method is based on ratio-spectra 1st derivative (1DD) spectrophotometry of the drug at 240 nm, over a concentration range of 5-35 μg mL−1 with mean percentage recovery of 99.77±0.974. The third method utilizes quantitative densitometric evaluation of thin-layer chromatography of rosuvastatin calcium in the presence of its oxidative degradation products, using ethyl acetate: methanol: ammonia (7:3:0.01, v/v/v) as a mobile phase. Chromatograms are scanned at 245 nm. This method analyses rosuvastatin calcium in a concentration range of 0.6-3.4 μgspot-1with mean percentage recovery of 99.78±1.419. The fourth method is an HPLC method for the simultaneous determination of rosuvastatin calcium in the presence of its oxidative degradation products. The mobile phase consists of water: acetonitrile: methanol (40: 40: 20 by volume). The standard curve of rosuvastatin calcium shows a good linearity over a concentration range of 10- 60 μg mL-1 with mean percentage recovery of 100.22±0.859. These methods were successfully applied to the determination of rosuvastatin calcium in bulk powder, laboratory-prepared mixtures containing different percentages of the degradation products and pharmaceutical dosage forms. The validity of results was assessed by applying standard addition technique. The results obtained were found to agree statistically with those obtained by a reported method, showing no significant difference with respect to accuracy and precision.

Download PDF
Selective Determination of Bambuterol Hydrochloride in the Presence of its Active Metabolite Terbutaline - 01/1

Amr Mohamed Mohamed Badawey

Amr M. Badawey, Nadia M. Mostafa, Abd El-Aziz B Abd El-Aleem and Nesrine T. Lamie

01/12/2011

Stability-indicative detn. of bambuterol hydrochloride (BH) in the presence of its degrdn. product (terbutaline), which is also the metabolite, is investigated. The degrdn. product was isolated, via acid-degrdn., characterized and elucidated. Selective quantification of BH, singly in bulk form, pharmaceutical formulations and/or in the presence of its major degrdn. product is demonstrated. The indication of stability was undertaken under conditions likely to be expected at normal storage conditions. Among the spectrophotometric methods adopted for quantification are second deriv. (2D), first deriv. of ratio spectra (1DD), ratio subtraction and bivariate anal. [on SciFinder(R)]

Download PDF
Stability Indicating PLS and PCR Chemometric Methods for the Determination of Rosuvastatin in Presence of its Two Oxidative Degradation Products - 01/1

Amr Mohamed Mohamed Badawey

Amr M. Badawey, Nadia M. Mostafa, Abd El- Aziz B. Abd El-Aleem, Nesrine T. Lamie

01/12/2011

Two multivariate calibration methods, including principal component regression (PCR) and partial least square (PLS), have been used for the determination of rosuvastatin in the presence of its oxidative degradation products. The PCR and PLS techniques are useful in spectral analysis due to the simultaneous inclusion of many spectral wavelengths instead of the single wavelength used in derivative spectrophotometry, thus a great improvement in the precision and predictive abilities of these multivariate calibrations is observed. A calibration set was constructed for the mixture and the best model was used for the prediction of the concentration of the selected drug. The proposed procedures were applied successfully in the determination of rosuvastatin in laboratory‐prepared mixtures and in commercial preparations. Rosuvastatin was analyzed with mean accuracies 99.93 ± 0.866 and 99.99±0.645 using the PCR and PLS methods respectively. The validity of the proposed methods was assessed using the standard addition technique. The proposed procedures were found to be rapid and simple and required no preliminary separation. They can therefore be used for the routine analysis of rosuvastatin in quality‐control laboratories.

Download PDF
Stability Indicating Spectrophotometric Methods for Determination of Rosuvastatin in the Presence of its Acid Degradation Products by Derivative Spectrophotometric Techniques - 01/1

Amr Mohamed Mohamed Badawey

Amr M. Badawey, Nadia M. Mostafa, Abd El Aziz B. Abd El- Aleem, Nesrine T. Lamie

01/11/2011

Two multivariate calibration methods, including principal component regression (PCR) and partial least square (PLS), have been used for the determination of rosuvastatin in the presence of its oxidative degradation products. The PCR and PLS techniques are useful in spectral analysis due to the simultaneous inclusion of many spectral wavelengths instead of the single wavelength used in derivative spectrophotometry, thus a great improvement in the precision and predictive abilities of these multivariate calibrations is observed. A calibration set was constructed for the mixture and the best model was used for the prediction of the concentration of the selected drug. The proposed procedures were applied successfully in the determination of rosuvastatin in laboratory‐prepared mixtures and in commercial preparations. Rosuvastatin was analyzed with mean accuracies 99.93 ± 0.866 and 99.99±0.645 using the PCR and PLS methods respectively. The validity of the proposed methods was assessed using the standard addition technique. The proposed procedures were found to be rapid and simple and required no preliminary separation. They can therefore be used for the routine analysis of rosuvastatin in quality‐control laboratories.

Download PDF

Follow us on

Visit the Faculty

ADS