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Staff Researches

Mennat Allah Ismail

Application of Chromatographic and Spectrophotometric Methods for The Analysis of Aliskiren and Hydrochlorothiazide Antihypertensive Combination.

Menatallah Ismail Ezzeldien Eissa

2013

Abstract ------------------------------------- High performance liquid chromatographic (HPLC) and spectrophotometric methods developed for the simultaneous determination of Aliskiren (ALK) and Hydrochlorothiazide (HCT) combination in bulk powder and in tablets dosage form. Determination of ALK and HCT was achieved by chromatographic separation on Econosphere C-18 column using a mobile phase consisting of water (pH 7.5): acetonitrile (50:50) at a flow rate of 0.5 mL.min−1 and UV detection at 208 nm. Method validation parameters were found to be acceptable over the concentrations range of 5-150 µg.mL-1, 1-50 µg.mL-1 for ALK and HCT respectively. Regarding the spectrophotometric methods, two methods were employed. Simultaneous Equation method, absorbance readings are taken at two wavelengths 277.48 nm (for ALK) and 267.48 nm (for HCT) in methanol. Dual Wavelength method, the difference between absorbance readings are taken at two wavelengths 273.3 nm, 260 nm (for Aliskiren) and 270 nm, 280 nm (for HCT) in methanol. The applied spectrophotometric methods were found to be rapid, specific, precise and accurate over the concentration range of 5 – 150 µg.mL-1 and 1 – 41 µg.mL-1 for ALK and HCT respectively and can be applied for the routine analysis of these drugs in bulk, and combined dosage form without any interference by the excipients.

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APPLICATION OF CHROMATOGRAPHIC AND SPECTROPHOTOMETRIC METHODS FOR THE ANALYSIS OF SELECTED ANTIHYPERTENSIVE COMBINATIONS.

Menatallah Ismail Ezzeldien Eissa

2013

ABSTRACT: High performance liquid chromatographic (HPLC) and spectrophotometric methods developed for the simultaneous determination of two antihypertensive mixtures namely(Perindopril, Indapamide ) and (Aliskiren , Hydrochlorothiazide ) in bulk powder and in tablets dosage form. In the first method, chromatographic separation of Perindopril and Indapamide was achieved on the Econosphere C-18 column (150mm x 4.6mm, 5µm) using a mobile phase system consisting of acetonitrile: 5mM sodium phosphate buffer (pH 7.5) (50:50) at a flow rate of 0.5 mL/min with UV detection at 202 nm. Method validation parameters were found to be acceptable over the concentration ranges of 1-140 μg/mL and 1-40 μg/mL for Perindopril and Indapamide respectively. Regarding the spectrophotometric methods , two methods were employed, Ratio Subtraction and First Derivative methods. In Ratio Subtraction method, absorbance readings are taken at two wavelengths 277.48 nm (λmax of Aliskiren) and 315 nm (extended spectrum of Hydrochlorothiazide) in methanol. In First Derivative method, absorbance readings are taken at two wavelengths 237.2 nm (for Aliskiren) and 275.8 nm (for Hydrochlorothiazide) in methanol. The applied spectrophotometric methods were found to be rapid, specific, precise and accurate over the concentration range of 5 – 150 μg/mL and 1 – 41 μg/mL for Aliskiren and Hydrochlorothiazide respectively. Keywords: High performance liquid chromatography; Ratio Subtraction; First Derivative; Perindopril; Indapamide; Aliskerin; Hydrochlorothiazide. Abbreviation: Aliskiren (ALK); Hydrochlorothiazide (HCT); Perindopril (PER); Indapamide (IND).

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FUE International Conference

Menatallah Ismail Ezzeldien Eissa

InterContinental CityStars

2012

FUE International Conference

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