Author : Amr Mohamed Badawy
CoAuthors : Mamdouh R.Rezk1*,AmrM.Badawy1,2, OsamaAbdel Sattar3, OmaimaM.Khattab4
Source : An Indian Journal
Date of Publication : 10/2016
Abstract :
Four stability-indicating methods were developed for determination of
tolterodine tartrate in the presence of its oxidative degradation product
(the metabolite). The degradation product was prepared via oxidation with
hydrogen peroxide. The degradation product was characterized and structurally
elucidated. The first method was the first derivative 1D by measuring
the peak amplitude at 292nm. The second method was a second derivative
by measuring the peak amplitude at 236, 287, 296 nm. The third
method was a high performance liquid chromatographic using a reversed
phase column and a mobile phase of phosphate buffer: methanol: triethyl
amine (60: 40; 0.2 by volume). The forth method was a thin layer chromatography
coupled with densitometric detection.
Selective quantification of tolterodine in pure form, pharmaceutical formulation
and/or in the presence of its degradant was demonstrated. The indication
of stability was done under condition likely to be expected at normal
storage condition.
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