Author : Marwa Tammam
CoAuthors : Marwa M. Raafat , Marwa Ali-Tammam and Amal E. Ali1,
Source : African Journal of Microbiology Research
Date of Publication : 10/2016
Abstract :
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen and a leading cause of hospitalacquired
infections. Characterization of the isolates from different infection sites might help to control
infections caused by the pathogen. The aim of the present work is to characterize P. aeruginosa
isolates recovered from different clinical specimens at two hospitals in Cairo with regard to their
antibiogram, genotypes and virulence factors. The highest antimicrobial resistance pattern was
exhibited by isolates from sputum. Resistance rate recorded for sputum samples to different in- use
antibiotics was 80, 80-100, 36, 54 and 54% for Penicillins, Cephems, Carbapenems, Aminoglycosides
and Fluoroquinolones, respectively. Phenotypic detection of virulence factors in P. aeruginosa isolates
included detection of protease, lecithinase, DNase, hemolysin and pyocyanin revealed that, each isolate
had at least one virulence factor. Protease and lecithinase were the most commonly detected, where 68
and 66% of the isolates showed positive protease and lecithinase activities respectively. Random
amplified polymorphic DNA (RAPD) genotyping using 2 random primers revealed 22 and 14 different
genetic profiles. Phylogenetic trees based on genetic distances showed 3 clusters with obvious
similarity between some isolates, indicating common sources of infection. No association could be
found between clustering pattern of the isolates, their antibiogram and virulence.
Key words: Pseudomonas aeruginosa, antibiogram, genotypes, random amplified polymorphic DNApolymerase
chain reaction (RAPD-PCR).
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